促红细胞生成素放射免疫分析及临床应用  

Radioimmunoassay of erythropoietin(EPO) and its clinical application

作　　者：王国洪[1] 许瑞吉[1] 赵技文[1] 张中书[1] 施益群[1] 

机构地区：[1]南京军区南京总医院核医学科,江苏南京210002

出　　处：《医学研究生学报》2000年第4期218-220,共3页Journal of Medical Postgraduates

摘　　要：目的 :建立促红细胞生成素 ( EPO)的放免分析方法 ,并探讨血清 EPO浓度测定的临床意义。　方法 :用rh EPO免疫动物制备了高质量的抗血清 ,建立了 EPO放免分析方法 ,并测定了正常人和贫血、肾衰患者血清 EPO值。　结果 :灵敏度为 0 .2 2 μg/L,批内 CV为 6.9%～ 8.4% ,批间 CV为 8.0 %～ 9.6% ,回收率为 93 .8%～110 .4%。正常血清 EPO值为 ( 1.5 9± 0 .69) μg/L,贫血患者为 ( 3 .64± 1.2 8) μg/L,肾衰患者为 ( 0 .85± 0 .2 5 ) μg/L,与正常人之间有显著性差异 ( P<0 .0 0 1)。　结论 :EPO的放免分析方法是一种简单、灵敏、特异的分析方法 ,可满足临床的需要 ,测定血清Objectives: To develop a method of radioimmunoassay for erythropoietin(EPO) determination and to explore its clinical value.　 Methods: ①Anti-recombinant EPO antibody with high affinity was prepared to develop the radioimmunoassay of EPO;②Serums EPO levels were measured in normal subjects and in patients with anemia and renal failure.　Results: The sensitivity was 0.22 μg／L,intra- and inter-assay variation were 8.0%～9.6％、6.9%～8.4％, respectively. Serums EPO levels in normal subjects and patients with anemia and renal failure were (1.59±0.69)μg／L、(3.64±1.28)μg／L and (0.85±0.25)μg／L, respectively; There were significant differences in serum concentrations of EPO among normal subjects and patients with anemia and renal failure (P＜0.001).　 Conclusions: This radioimmunoassay of EPO fulfills reasonable requirements of a clinical routine in terms of specificity, sensitivity and practicability. Measurement of EPO concentration is useful for clinical diagnosis and choice of treatment of anemia and renal failure.

关 键 词：放射免疫分析 促红细胞生成素 贫血 肾衰 

分 类 号：R446.61[医药卫生—诊断学]