肝树突状细胞与骨髓树突状细胞体外生物免疫特性的比较研究  

In vitro COMPARATIVE RESEARCH OF DENDRITIC CELLS DERIVED FROM LIVER AND BONE MARROW

作　　者：傅继东[1] 钟翠平[1] 王国强[1] 范强[1] 顾云娣[1] 张新华[1] 

机构地区：[1]上海医科大学组织学胚胎学教研室,200032

出　　处：《解剖学报》2000年第z1期81-84,144,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(39770387)

摘　　要：目的 探讨肝树突状细胞（ｈｅｐａｔｉｃ　ｄｅｎｄｒｉｔｉｃ　ｃｅｌｌ，ＨＤＣ）的生物主疫功能及在肝移植耐受中的作用。方法 分别从肝脏和骨髓分离、培养ＤＣ，以免疫组织化学及流式细胞术检测两种ＤＣ生物特性及免疫活性的差异。结呆 培养６～８ｄ，骨髓 ＤＣ（ＢＭ－ＤＣ）有大量细胞从集落中释放，并呈成熟状态，细胞表面组织相容性抗原 ＭＨＣ ｃｌａｓｓ、ＭＨＣ ｃｌａｓｓ Ⅱ及共刺激分子ＣＤ８０（Ｂ７－１）、ＣＤ８６（Ｂ７－２）都有较高水平表述，混合淋巴细胞反应（ＭＬＲ）中表现出有强烈刺激Ｔ细胞增生的能力；而同时期的ＨＤＣ具有非成熟ＤＣ的形态及功能特征，表面分子表达很低，无刺激Ｔ细胞增生的能力。结论 实验结果提示ＨＤＣ具有ＢＭ－ＤＣ不同的生物特性可能是产生肝脏移植受特性的一个重要原因。Objective To investigate the biological character istics of hepatic de ndritic cell(HDC) and its functions in the immune tolerance of hepatic allograft s. Methods DC progenitors were respectively isolated from liver and bone marrow o f C57BL/6 J mice, and were propagated in liquid culture with GM-CSF. The immune fu nction was detected by using immunochemical staining, FCM and MLR. Resu lts Afte r 6-8 days' culture, most BM-DC released from proliferating aggregates. They e xp ressed high level MHC classⅡ and costimulating molecules CD80 (B7-1),CD86(B7- 2),and the ability for strongly stimulating T cell proliferation, which showed the stro ng mature features. But HDC expressed lower level of those molecules, and failed in inducing primary allogeneic responses of naive T cells, Conclusion These obs ervations demonstrated that liver-derived DCs have the bio-function of immatur e DC, which may contribute to the immune tolerance of hepatic allografts.

关 键 词：肝脏 树突状细胞 免疫耐受 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学]