卵巢癌抗独特型疫苗(6B11mGM)动物体内诱导抗肿瘤免疫应答的实验研究  

STUDY OF THE ANTITUMOR IMMUNE RESPONSES INDUCED in vivo BY ANTI-IDIOTYPIC VACCINE OF OVARIAN CANCER (6B11mGM)

作　　者：崔恒[1] 刘蓓[2] 冯捷[1] 昌晓红[1] 叶雪[1] 李艺[1] 曹善津[1] 付天云[1] 姚煜[1] 钱和年[1] 

机构地区：[1]北京大学人民医院妇科肿瘤中心,北京100044 [2]天津市中心妇产科医院实验室,天津300052

出　　处：《解剖学报》2000年第z1期101-105,共4页Acta Anatomica Sinica

基　　金：教育部优秀年轻教师基金资助项目

摘　　要：目的 观察卵巢癌抗独特型单链抗体 ６Ｂ１１ＳｃＦｖ与鼠粒细胞巨噬细胞集落刺激因于（ｍｕｒｉｎｅ ｇｒａｎｕｌｏｃｖｔｅ－ｍａｃｒｏｐｈａｇｅ ｃｏｌｏｎｙ－ｓｔｉｍｕｌａｔｉｎｇ ｆａｃｔｏｒ．ｍＧＭ－ＣＳＦ）的融合蛋白（６Ｂ１１ｍＧＭ）在小鼠体内诱导抗肿瘤免疫反应情况，探讨此种融合蛋白作为卵巢癌疫苗的可能性及适当的免疫方法。方法 用融合蛋白６Ｂ１１ｍＧＭ免疫ＢＡＬＢ／ｃ小鼠，制备抗血清。采用间接ＥＬＩＳＡ及免疫流式方法分析抗血清特征。结果 经ＥＬＩＳＡ检测，融合蛋白６Ｂ１１ｍＧＭ免疫小鼠后，可诱导机体产生ＡＢ３。同时可刺激小鼠脾脏淋巴细胞ＣＤ４＋细胞明显升高，ＣＤ８＋细胞略有升高。结论 融合蛋白６Ｂ１１ｍＧＭ可诱导机体产生特异体液免疫和细胞免疫反应，为抗独特型卵巢癌疫苗用于临床奠定了基础。Objective To observe whether antitumor immune response c an be induced in BALB/c mice immunized with a fusion protein(6B11mGM) of anti-idiotypic single chain antibody (6B11scFv) and murine granulocyte-macrophage colony-stimulating factor (GM-CSF) instead of ovarian carcinoma antigen.Methods BALB/c mice were immuni zed repeatedly with 6B11mGM.Indirect ELISA test and immune flow cytometer were used for analyzing the characterization of anti-idiotypic ScFv(Ab3).Result s Ab3 could be detected in the sera of immunized mice with 6B11mGM by ELISA test .Moreover,it stimulated CD4+T cell from the spleen of BALB/c mice to proliferate and CD8+ T cell to proliferate slightly.Conclusion 6B11mGM probably induce b oth humoral and cellular immunity against ovarian carcinoma in vivo .It illus tra ted a strong basic for using fusion protein 6B11mGM as anti-idiotype vaccines a gainst ovarian carcinoma.

关 键 词：卵巢癌 抗体 抗独特型 肿瘤疫苗 动物 实验 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学]