GnRH类似物阿拉瑞林对培养的胃平滑肌细胞内钙离子浓度变化的影响  

EFFECTION OF GnRH ANALOGUE ON INTRACELLULAR CALCIUM MOBILIZATION IN CULTURED RAT STOMACH SMOOTH MUSCLE CELLS

作　　者：陈蕾[1] 黄威权[1] 刘丽宏[2] 吕葆真[1] 蒲若蕾[1] 

机构地区：[1]第四军医大学组织学胚胎学教研室,西安710032 [2]第四军医大学药理学教研室,西安710032

出　　处：《解剖学报》2000年第z1期112-114,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(39770388)

摘　　要：目的 研究ＧｎＲＨ类似物（阿拉瑞林）诱导胃平滑肌细胞内钙动员的机制。方法 采用共聚焦技术检测细胞内钙荧光强度的变化。结果 （１）在Ｈａｎｋｓ液中，ＧｎＲＨ类似物浓度为１０７、１０６、１０－５ｍｏｌ－Ｌ．－１时，胃平滑肌细胞内Ｃａ２＋逐渐升高，其峰高（ｐｅａｋ－ｒｅｓｔｉｎｇ）分别为 ６．００ ± ０．５０、９．２３± ０．６２、１８．９７± ２．４２，其达峰时间分别为 ４２．３７± １．６５、５４．９３± ３．２２、１０５．６３ ± ３．９２ｓ，井呈时间和剂量依赖性；（２）当阿拉瑞林浓度为１０－４ｍｏｌ·Ｌ－１时，胞内Ｃａ２＋略有升高，与１０－５ｍｏｌ·Ｌ－１相比（Ｐ＜０．０１）；（３）在有外钙和无外钙时，阿拉瑞林对胞内Ｃａ２＋的升高亦有显著差异（Ｐ＜０．０１）；（４）在无外钙并加入内罗陡孵育后，ＧｎＲＨ引起的胞内 Ｃａ２＋轻度升高，但与 Ｄ－Ｈａｎｋｓ组相比无显著性差异（Ｐ＜０．０５）；（５）在 Ｈａｎｋｓ液中，用拉西地平孵育后，再加入阿拉瑞林，其胞内Ｃａ２＋仍可升高，井有显著差异（Ｐ＜０．０１）。结论Ｃａ２＋参与了ＧｎＲＨ类似物阿拉瑞林对胃平滑肌细胞作用的细胞内分子转导过程。细胞内Ｃａ２＋的增加既源于内钙的释放又源于外?Objedctive　To study the mechanism of GnRH agal ogus( Alarelin)on［Ca2+］i mobilization in stomach smooth muscle cells (SSMC)o f rats.Methods Cells were cutured and loaded with Fluo -3-AM.［Ca2+］i was measured by fluorescent intensity(FL)in each cell with confocal microscopy.Results (1)In Hanks solution，10 - 7，10-6，10 -5mol*L-1Alarelin can elevate［Ca2+］i ， its peak-resting values reached 6.00±0.50、9.23±0.62、18.97±2.42 respec tively，which indicate that the level of ［Ca2+］i act in an dose-depen de nt and time-dependent.(2) Contrarily，when Alarelin was 10-4mol*L-1 ， its peak-resting value only reached 6.32±0.67， conpared with Alarelin 10 -5mol*L-1，which was sigificantly lower(P＜0.01.(3)［ Ca2+］i wa s also singificantly higher in Hanks solution than that in D-Hanks solution(P＜ 0.05).(4)In D-Hanks solution with ryanodine，Alarelin had no effect on levels of ［Ca2+］i，compared with group of D-Hanks(P＞0.05).( 5)When pertreatment with Lacidipine in Hanks solution，the effect of Alarelin 10-5mol*L -1 was partly inhibited(P＜0.01)。Conclusion The data indicate that［Ca2+］i plays an important role in molecular mechanism of intrace llular singal transduction pathways which GnRH analogue，Alarelin mediate in SSM C .T he increasing of［Ca2+］i was caused by releasing from intracellular sto r es and extracellular Ca2+influx，possibly through L-type calcium channels .

关 键 词：GNRH类似物 胃平滑肌 钙 Fluo-3-AM 共聚焦显微 镜检查 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学]