催化过氧化氢氧化钙黄绿素褪色光度法测定痕量铁  

Determination of Trace Iron by Catalytic Spectrophometric Based on Fe(Ⅲ)-H_2O_2-DBS-Calcein

作　　者：李文琦[1] 戴鸿[1] 

机构地区：[1]漳州师范学院化学系,福建漳州363000

出　　处：《漳州师范学院学报（自然科学版）》2004年第2期79-81,78,共4页Journal of ZhangZhou Teachers College（Natural Science)

摘　　要：基于Fe(Ⅲ)对过氧化氢氧化钙黄绿素的催化反应，建立了高灵敏测定痕量铁的新方法本法线性测定范围为0．4-4ig/L，检出限为1．4×10^-10g／mL，线性回归方程为△A=-0．0033+0．03812CFe3+(ig，L)，相关系数r=0．9991．本法已用于人发、茶叶中铁含量的测定。A new kinetic spectrophotometric method for the determination of trace Fe(Ⅲ)has been developed. Its based on the discoloring oxidation between calcein and hydrogen peroxide catalyzed by Fe(Ⅲ).The linear range is 0.4~4g/L with a detection limit of 1.4х10-10g/mL,and the regression equation of working curve isΔA=-0.0033+0.03812CFe3+ (靏/L), correlation coefficient r=0.9991.The method has been applied to the determination of iron in hair sample and tea sample with satisfactory results.

关 键 词：催化 过氧化氢 氧化钙 黄绿素 褪色光度法 测定 痕量铁 微量元素 

分 类 号：Q581[生物学—生物化学] O657.3[理学—分析化学]