消癥颗粒对大鼠子宫肌瘤的干预作用及相关机制的研究  

STUDY ON INTERVENTION OF XIAOZHENG GRANULE ON RATS’ UTERINE LEIOMYOMA AND ITS MECHANISM

作　　者：金国梁[1] 周大兴[1] 张艳[1] 胡军[1] 张要栋[1] 李娟[1] 

机构地区：[1]浙江中医药大学,杭州310053

出　　处：《中国中医药科技》2009年第3期171-173,共3页Chinese Journal of Traditional Medical Science and Technology

基　　金：浙江省科技厅项目No.2004C33002

摘　　要：目的:观察消癥颗粒对大鼠实验性子宫肌瘤生长的抑制作用,并探讨其相关作用机制。方法:采用注射己烯雌酚和黄体酮进行子宫肌瘤造模,对造模大鼠分别给予消癥颗粒、桂枝茯苓胶囊和三苯氧胺,观察比较各组大鼠子宫大体解剖形态学和光镜下病理形态改变,并检测血清免疫因子IL-2、TNF-α水平及抗细胞凋亡因子Bcl-2的表达水平。结果:造模大鼠均出现较明显的子宫平滑肌瘤样改变,消癥颗粒各剂量组大鼠子宫肌瘤样增生则均有不同程度的减轻;与模型组相比,其血清IL-2水平升高(P<0.01或P<0.05),TNF-α水平(P<0.01)降低,尤以消癥颗粒高剂量组最为明显;在细胞凋亡调控方面,尚能下调Bcl-2蛋白的表达水平,与模型组相比,差异均具有显著性(P<0.01)。结论:消癥颗粒对于大鼠实验性子宫肌瘤生长具有一定的抑制作用,调整并改善机体的免疫功能、抑制抗凋亡基因Bcl-2的表达水平可能是其防治子宫肌瘤的主要作用机理之一。Objective:To observe the Inhibition of xiaozheng granule on rats’ uterine leiomyoma and discuss its mechanism.Methods: Models with uterine leiomyoma were made by injecting diethylstilbestrol and progesterone.All models were divided into xiaozheng granule three dose groups and Chinese and western medicine control groups, meanwhile the normal group was set. Each group rats were given correspending medicines,xiao zheng granule 1.6,2.8,5.6g/kg,guizhi fuling capsule 0.5g/kg,tamoxifen 0.25 mg/kg and NS for 12 weeks. The gross anatomic characterastic and pathological changes of rats’ uterus were observed, serum IL-2 and TNF-α levels were tested by ELISA, Bcl-2 expression was detected by immunohistochemical method.Results: All modelled rats showed tumor-like lesions obviously, theses changes were improved in each treated group. IL-2 level was obviously raised in the treated groups and TNF-α was lowerd, these changes in the high dose xiaozheng granule group were the most obvious. Bcl-2 expression was down-regulating significantly in each treated group.Conclusion:Xiaozheng granule can inhibit the growth of uterine leiomyoma, regulating the immune function and inhibiting Bcl-2 expression maybe its main mechanism.

关 键 词：子宫肌瘤/中医药疗法 @消癥颗粒/治疗应用 子宫肌瘤/病理学 白细胞介素2/血液 肿瘤坏死因子/血液 基因表达 大鼠 

分 类 号：R285.5[医药卫生—中药学]