镧对大鼠学习记忆及大脑皮质c-fos mRNA和c-Fos蛋白表达的影响  

Effects of lanthanum on memory and expression of c-fos mRNA and c-Fos protein of cerebral cortex in rats

作　　者：杨敬华[1] 蔡原[2] 刘秋芳[2] 张立丰[2] 齐鸣[2] 巫生文[2] 鲁帅[2] 程铭[2] 

机构地区：[1]中国医科大学公共卫生学院环境卫生学教研室,沈阳110001 [2]中国医科大学公共卫生学院卫生毒理学教研室

出　　处：《卫生研究》2009年第3期348-351,共4页Journal of Hygiene Research

基　　金：辽宁省科技厅基金资助项目(No.2005225008-3)

摘　　要：目的研究镧对学习记忆的影响,并从大脑皮质即刻早期基因表达的角度探讨镧影响学习记忆的机制。方法40只成年雌性Wistar大鼠随机分成对照组和低、中、高剂量染镧组,染镧组仔鼠在出生至断乳后1个月期间染镧,然后进行神经行为学测试(水迷宫和穿梭箱测试),测定大脑皮质中c-fosmRNA和c-Fos蛋白以及尼氏体表达情况。结果神经行为学测试结果显示染镧组仔鼠的学习记忆能力明显低于对照水平,呈现一定的量-效关系。低剂量染镧组仔鼠大脑皮质c-fosmRNA和c-Fos蛋白表达水平明显低于对照水平,中剂量染镧组仔鼠海马c-fosmRNA表达水平明显低于对照水平,c-Fos蛋白表达水平明显低于对照和低剂量染镧组,高剂量染镧组仔鼠大脑皮质c-fosmRNA和c-Fos蛋白表达水平均明显低于对照和低剂量染镧组。另外,低剂量染镧组大脑皮质神经细胞中尼氏体表达水平明显低于对照水平,中、高剂量染镧组大脑皮质神经细胞中尼氏体表达水平进一步减少。结论染镧造成学习记忆能力下降,这可能与染镧引起大脑皮质即刻早期基因的基因和蛋白表达水平下降、神经细胞功能削弱有一定关系。Objective To investigate the effects of lanthanum on memory, and explore the corresponding mechanism from immediate early gene expression of cerebral cortex in rats. Methods Female adult rats were divided into control, low, middle, and high dose lanthanum groups at random. Offspring of lanthanum groups were administrated with lanthanum from birth to weaning for one month. Ability of memory of offspring was detected by neuroethology testing. The expression levels of c-fos mRNA, c-Fos Protein and Nissl body in cerebral cortex of offspring were also measured. Results The data in neuroethology testing showed that memory of offspring in lanthanum groups were significantly lower than control level and presented dose-effect relationship to some extent. The expression levels of c-fos mRNA and c-Fos protein in cerebral cortex of low dose lanthanum group were lower significantly than control values. As to middle dose lanthanum group, the expression levels of c-fos mRNA in cerebral cortex were lower significantly than control values, and c-Fos protein expression were lower significantly than control and low dose lanthanum group. Both c-fos mRNA and c-Fos protein expression in cerebral cortex of high dose lanthanum group decreased significantly as compared with control and low dose lanthanum group. In addition, the expression levels of Nissl body in cerebral cortex of low dose lanthanum group were lower significantly than control values. The expression levels of Nissl body in cerebral cortex of middle and high dose lanthanum group decreased further. Conclusion Lanthanum induced deficit in memory, which is possibly related to decreased levels of immediate early gene expression and neuronal function in cerebral cortex caused by lanthanum.

关 键 词：镧 学习记忆 大脑皮质 蛋白表达 环境毒理 

分 类 号：R994.6[医药卫生—毒理学] O593.2[医药卫生—药学] R338.6[理学—应用物理]