氢醌对人L-02肝细胞泛素结合酶Rad6B表达的影响  

Effect of hydroquinone on the expression of ubiquitin-conjugating enzyme Rad6B in human L-02 hepatic cells

作　　者：胡恭华[1] 庄志雄[1] 杨建平[1,2] 黄海燕[1,3] 杨淋清[1,3] 

机构地区：[1]赣南医学院预防医学系,赣州341000 [2]深圳市宝安区疾病预防控制中心职业病防治所 [3]深圳市疾病预防控制中心毒理研究室

出　　处：《卫生研究》2009年第3期352-354,共3页Journal of Hygiene Research

基　　金：国家重点基础研究发展计划(973计划)项目(No.2002CB512903;No.2002CB512904)

摘　　要：目的研究氢醌(HQ)对人L-02肝细胞中泛素结合酶Rad6B表达的影响。方法将L-02肝细胞用不同浓度(0、5、10、20、40、80和160μmol/L)的HQ处理24h之后,采用实时荧光定量PCR技术检测Rad6B在mRNA水平上的表达;采用Western blot方法检测Rad6B在蛋白质水平上的表达。结果在0～160μmol/L的范围内,HQ可诱导Rad6B在mRNA和蛋白质水平上表达的增加,具有随着剂量的增加而升高的趋势;并且各染毒剂量组(5、10、20、40、80和160μmol/L组)肝细胞Rad6B在mRNA和蛋白质水平上的表达均明显地高于对照组(0μmol/L组)(P<0.01);当HQ的作用浓度达到160μmol/L时,Rad6B的表达水平达到最大值,其mRNA和蛋白质表达的相对定量值分别为4.35和0.85。结论HQ可使L-02肝细胞的Rad6B表达增加。Objective To study the effects of hydroquinone(HQ) on expression of ubiquitin-conjugating enzyme Rad6B in human L-02 hepatic cells. Methods After L-02 hepatic cells were at the concentrations of 0,5,10,20,40,80,and 160μmol/L HQ for 24h,and the levels of Rad6B mRNA and protein expressions in L-02 hepatic cells were detected by real-time fluorescent quantitative polymerase chain reaction(QPCR) technique and Western blot method respectively. Results It was found that HQ in the range of 0-160μmol/L could induce increases in the expressions of Rad6B mRNA and protein, which was in a dose-dependent manner. The levels of Rad6B mRNA and protein expressions in the treated group of 5,10,20,40,80,and 160μmol/L were higher than those in the control(P<0.01), levels of Rad6B mRNA and protein expressions reached the maximun when L-02 hepatic cells were treated by HQ at the concentrations of 160μmol/L,and the relative quantity values were 4.35 and 0.85,respectively. Conclusion HQ could regulate up the expression of Rad6B in L-02 hepatic cells.

关 键 词：氢醌 L-02肝细胞 Rad6B 实时荧光定量PCR 泛素结合酶 

分 类 号：R994.6[医药卫生—毒理学] R114[医药卫生—药学] Q593.2[生物学—生物化学]