荧光分析法测定枸杞中维生素C  

Determination of Vitamin C in Chinese Wolfberry by Fluorescence Spectrophotometry

作　　者：崔香[1] 寇雪玲[2] 

机构地区：[1]青海师范大学化学系,青海西宁810008 [2]中石化勘探南方分公司研究院,四川成都610041

出　　处：《青海师范大学学报（自然科学版）》2009年第2期58-60,共3页Journal of Qinghai Normal University(Natural Science Edition)

摘　　要：本文建立了荧光法快速测定枸杞中维生素C的方法.样品经草酸提取,活性炭氧化后,与邻苯二胺反应,荧光化合物激发波长为343nm,发射波长为425nm.维生素C在3-20mg.L-1浓度范围内测定呈现良好线性关系.The paper establishes a kind of rapid determination method of Vitamin C in Chinese wolfberry by fluorescence spectrophotometer.After extracted by oxalic acid,oxidized by actived carbon,the samples react with o-phenylenediamine,and the excitated wavelength of the compound of fluorescence is 355nm,the emission wavelength is 425nm.Its luminescence intensity is rigid linear to Vitamin C 's concentration between 2 and 20 mg·L-1.

关 键 词：荧光法 枸杞 维生素C 邻苯二胺 

分 类 号：O657.39[理学—分析化学]