乙醇对大鼠下丘脑促性腺激素释放激素神经元功能的影响  

Influence of Alcohol to GnRH Neurons in Rats' Hypothalamus

作　　者：李鑫[1] 王晓萃[2] 程秀臻[1] 

机构地区：[1]潍坊医学院生理学教研室,山东潍坊261053 [2]潍坊医学院解剖学教研室

出　　处：《潍坊医学院学报》2009年第2期81-83,Ⅰ,共4页Acta Academiae Medicinae Weifang

基　　金：山东省教育厅资助课题(课题编号:J05L07)

摘　　要：目的研究乙醇对雄性大鼠下丘脑促性腺激素释放激素神经元(GnRH neurons)合成和释放激素功能的影响。方法健康Wistar雄性大鼠40只,随机分为4组:对照组、低浓度乙醇组、中浓度乙醇组和高浓度乙醇组。在灌服不同浓度的乙醇后2h,采用酶法检测血中乙醇浓度(blood alcohol concentration,BAC);免疫组织化学法观察下丘脑促性腺激素释放激素(Gonadotropin-releasing hormone,GnRH)神经元密度和染色反应的变化。结果与对照组相比,随着灌胃乙醇浓度的升高,各实验组大鼠BAC值逐渐升高,并且均已达到乙醇中毒的标准(500～1000mg/L);高浓度乙醇组大鼠下丘脑内GnRH阳性神经元密度和染色强度较对照组明显减小(P<0.01),中、低浓度乙醇组下丘脑内GnRH阳性神经元密度和染色强度较对照组变化不显著(P>0.05)。结论急性乙醇中毒雄性大鼠,下丘脑神经递质GnRH神经元合成和分泌功能受抑,这可能与乙醇对生殖行为的抑制有关。Objective To study the influence of alcohol on the function of GnRH neurons in rat hypothalamus.Methods Forty healthy male Wistar rats were randomly divided into the control group,low concentration group,middle concentration group and high concentration group.The rats were induced with alcohol solution.After 2 hours,the following indexes were detected:①Blood alcohol concentration (BAC) with enzymatic method;②Changes of Gonadotropin-releasing hormone(GnRH) neuronal density and staining reaction in hypothalamus. Results ①Contrasted with the control group,as the alcohol concentration increased,BAC of each experimental group increased and achieved the criterion for alcoholism (500～1000mg/L);②The GnRH positive neuronal density and staining reaction in middle and low alcoholic concentration groups in hypothalamus had no significant changes (P>0.05),yet the density and staining reaction of high alcoholic concentration group were induced obviously (P<0.01).Conclusion In acute alcohol intoxication,the inhibition of alcohol to male rats' reproduction behavior might be relevant to the function of GnRH neurons in hypothalamus.

关 键 词：乙醇 下丘脑 促性腺激素释放激素神经元 

分 类 号：R338[医药卫生—人体生理学]