人脐血细胞移植对高血糖模型小鼠的治疗作用  

Therapeutic Effect of Human Umbilical Cord Blood Cells on Diabetic Mice

作　　者：张芳婷[1] 万汇涓[1] 叶静[1] 房家智[1] 尹美珺[1] 黄春桥[1] 于洁[1] 

机构地区：[1]北京大学深圳医院中心实验室,广东省深圳市518036

出　　处：《中国全科医学》2009年第16期1492-1494,共3页Chinese General Practice

基　　金：深圳市科技计划重点项目(200801001)

摘　　要：目的探讨尾静脉移植人脐血细胞对糖尿病模型小鼠血糖的影响。方法选择60只昆明小白鼠,随机抽取5只作为对照组,其余55只采用腹腔注射链脲佐菌素(STZ)的方法制备糖尿病鼠模型。成模后将小鼠随机分成移植组(26只)和糖尿病组(12只)。将分离的人脐血单个核细胞于对数增长期用PKH26标记后,以2×106/ml经尾静脉注射入移植组小鼠体内。于注射后第4天和第14天观察3组小鼠的血糖和胰岛素水平,并用流式细胞仪和荧光显微镜观察PKH26标记的人脐血单个核细胞在体内的分布。结果人脐血单个核细胞植入第4天,移植组小鼠血糖和胰岛素水平与糖尿病组、对照组比较,差异均有统计学意义(P<0.05);移植第14天移植组小鼠血糖水平及胰岛素水平与糖尿病组比较,差异均无统计学意义(P>0.05)。流式细胞检测发现移植第4天的糖尿病小鼠的胰、脾、肝、骨髓组织内均可见PKH26+细胞,而移植第14天上述各部位未发现PKH26+细胞,荧光显微镜观察上述部位的冷冻切片结果也支持这一结果。结论将人脐血细胞移植到未用免疫抑制剂的糖尿病小鼠体内可存活,并可以在一定时间内降低糖尿病小鼠的血糖水平。Objective To observe the therapeutic effect of human umbilical cord blood cells transplanted via caudal vein on blood glucose in diabetic mice.Methods Sixty Kunming mice were enrolled,5 of which were chosen as control group,the others given intraperitoneal injection of streptozotocin(STZ) to prepare diabetic mouse models.The prepared models were randomized into groups transportation (n=26) and diabetes (n=12).The isolated umbilical cord blood mononuclearcells were labeled by PKH26 in increased logarithmic phase,and then injected,in 2×106/ml,via caudal vein,into transportation group.The levels of serum glucose and insulin were determined on days 4,14 after injection,and the distribution of PHK26-labeled umbilical cord blood mononuclearcells were observed by flow cytometry and fluorescence microscopy.Results On day 4 after transportation,the serum glucose levels decreased significantly(P<0.05),but insulin levels increased when compared with pre-transplantation(P<0.05).On day 14,serum glucose returned to pre-transplantation level,and insulin level decreased as compared with level on day 4(P<0.05),not significantly different from control.PKH26 positive cells were found in pancreases,spleen,liver and bone marrow on day 4 after transportation,but not on day 14.The frozen section in these parts supported these results by fluorescence microscopy.Conclusion Human umbilical cord blood cells,which can survive in diabetic mice without immunosuppressive agents ,can reduce blood glucose levels in a certain period of time.

关 键 词：脐血干细胞移植 高血糖小鼠 血糖 

分 类 号：R587.1[医药卫生—内分泌]