牛尾山药离体再生体系的建立初探  

The Establishment of in vitro Regeneration System for Dioscrea batatas

作　　者：唐虹[1] 吴佳丽[1] 张领[1] 鲍菊[1] 韩伍渠 娄平[2] 

机构地区：[1]贵州省安顺市农业科学研究所,贵州安顺561000 [2]贵州省紫云县农业局,贵州紫云550800

出　　处：《贵州农业科学》2009年第9期31-33,共3页Guizhou Agricultural Sciences

基　　金：安顺市年度科技攻关项目"山药试管苗组培快繁技术研究"(2007102)

摘　　要：采用安顺刘官牛尾山药茎段和顶芽为外植体,研究了不同激素对山药再生体系建立的影响.结果表明:以MS为基本培养基幼嫩茎段在MS+6-BA 1.0 mg/L+NAA 0.3 mg/L的培养基中,可直接形成根系发达、健壮的山药组培苗,顶芽在6-BA 4.5 mg/L、NAA 2.0 mg/L的MS培养基中愈伤诱导效果最好,以MS+NAA 0.5 mg/L+6-BA 1.0 mg/L培养基适于丛生芽分化,以0.3 mg/L NAA与0.3 mg/L 6-BA的培养基有利试管苗增殖,生根以加NAA 0.2～0.3 mg/L的MS培养基生根效果最佳,生根率达90%以上.Stem segments and apical buds of Dioscrea batatas in Liuguan Township,Anshun district were used as explants to study the effect of different hormone on the establishment of in vitro regeneration system.The results showed that the young stem segment could develop to healthy plantlet with well-developed root system on MS+6-BA 1.0 mg/L + NAA 0.3 mg/L media based on MS basic media,apical bud had best callus induction in MS + 6-BA 4.5 mg/L + NAA 2.0 mg/L,MS + 6-BA 1.0 mg/L+ NAA 0.5 mg/L was suitable for differentiation for clustered buds,6-BA 0.3 mg/L+ NAA 0.3 mg/L was suitable for plantlet propagation,and MS + NAA 0.2~0.3 mg/L was best for rooting with 90% of rooting rate.

关 键 词：山药 离体培养 组培苗 再生体系 

分 类 号：S631.2[农业科学—蔬菜学]