高效液相色谱法测定硒-甲基硒代半胱氨酸  

Determination of Se-methylselenocysteine by HPLC

作　　者：刘建群[1] 刘芳[2] 张锐[1] 张小平[3] 寇晓莉[1] 

机构地区：[1]江西中医学院现代中药制剂教育部重点实验室,江西南昌330004 [2]江西萍乡高等专科学校化工系,江西萍乡337000 [3]江西川奇药业有限公司,江西南昌330029

出　　处：《萍乡高等专科学校学报》2009年第3期59-62,共4页Journal of Pingxiang College

摘　　要：建立硒-甲基硒代半胱氨酸(SeMC)测定方法,优选合成工艺,控制成品质量。采用高效液相色谱法(HPLC),测定条件为:色谱柱为HypersilC4(4.6mm×300mm,5μm),流动相为水(用磷酸调至pH2.50)-甲醇(95:5),检测波长235nm,流速1.0ml/min,柱温25℃。结果:硒-甲基硒代半胱氨酸的线性范围为0.08μg～2μg,r=0.9995,高、中、低三种不同浓度的平均加标回收率范围为99.97%～100.62%,RSD为0.53%～0.88%。结论:该法操作便捷,结果准确,适合合成工艺评价和成品质量控制。Objective :To establish the assay of Se-methylselenocysteine for technology and quality control.Methods:A HPLC method was established with the follow measurement conditions.Hypersil C4 column was used with water(adjust pH to 2.50 with phosphoric acid)-MeOH(95:5)as the mobile phase.The content was detected at wavelength of 235nm and at temperature of 25℃.The flow rate was at 1.0ml/min.Results:The calibration curve was linear in the range of 0.08μg-2μg,r=0.9995.The recovery range for three concentrations of h...

关 键 词：硒-甲基硒代半胱氨酸 含量测定 高效液相色谱法 

分 类 号：R927[医药卫生—药学]