呱氨托美汀与牛血清白蛋白的相互作用  

Interaction of Bovine Serum Albumin with Amtolmetin Guacyl

作　　者：张爱丽 杨冉[1] 屈凌波[1,2] 

机构地区：[1]郑州大学化学系,郑州450001 [2]河南工业大学化学化工学院,郑州450001

出　　处：《郑州大学学报（理学版）》2009年第2期95-98,共4页Journal of Zhengzhou University:Natural Science Edition

基　　金：国家自然科学基金资助项目,编号20472076;河南省杰出青年科学基金资助项目,编号0512001400

摘　　要：研究药物小分子呱氨托美汀与生物靶分子牛血清白蛋白的相互作用机制.应用荧光猝灭法,研究了不同温度和酸碱度之下二者的结合作用.Stern-Volmer方程计算结果表明,在弱酸、弱碱及中性条件下,呱氨托美汀均以静态猝灭的方式猝灭牛血清白蛋白的内源荧光;几种金属离子在中性环境下对2者的作用有微弱影响;呱氨托美汀小分子与生物靶分子牛血清白蛋白主要以静电作用方式相结合.The interaction mechanism of bovine serum albumin(BSA) with amtolmetin guacyl(AMG) is studied using fluorescence quenching method.The binding constants at different temperatures are determined and the quenching mechanism of them is suggested as static quenching.The effect of various metal ions on the binding constants is also discussed.The interaction between AMG and BSA is strong and the binding force is mainly electrostatic force.

关 键 词：荧光光谱 呱氨托美汀 牛血清白蛋白 

分 类 号：R96[医药卫生—药理学]