血小板源性生长因子介导内皮素-1所致人肾小球细胞外基质的合成  

Platelet-derived growth factor-B mediates human glomerural mesangial cell extracellular matrix excretion induced by endotelin-1

作　　者：赵晋晋[1] 雷闽湘[1] 周素娴[1] 蒋铁建[1] 

机构地区：[1]中南大学湘雅医院内分泌科,长沙410008

出　　处：《山东大学学报（医学版）》2009年第7期9-12,共4页Journal of Shandong University:Health Sciences

摘　　要：目的探讨内皮素-1(ET-1)致系膜细胞细胞外基质(ECM)合成增加的介导途径。方法体外培养人肾小球系膜细胞,按培养条件不同分为正常对照组、ET-1组、阻断剂(RG50864)干预组及氯沙坦干预组,分别培养24 h。采用ELISA法测定细胞上清液中IV型胶原(Col IV)、纤维连接蛋白(Fn)的含量;RT-PCR法测定培养系膜细胞内血小板源性生长因子-B(PDGF-B)mRNA的表达,Western blot法检测PDGF-B蛋白的表达。结果①在外源性ET-1作用下,上清液中Col IV、Fn蛋白含量、系膜细胞PDGF-B mRNA及蛋白表达增加(P<0.05),以10-7mol/L ET-1作用最为明显。RG50864呈浓度依赖方式抑制ET-1所致的Col IV、Fn分泌增加(P<0.05);②氯沙坦能抑制ET-1所致系膜细胞PDGF-B mRNA及蛋白表达增加,减少上清液中Col IV、Fn蛋白的含量(P<0.05)。结论①ET-1能够刺激ECM分泌增加并促进系膜细胞合成PDGF-B,ET-1所致的系膜细胞ECM分泌增加可能部分由PDGF-B介导;②氯沙坦可以通过拮抗系膜细胞PDGF-B的产生进而减少ECM的分泌和沉积,从而产生明显的肾脏保护作用。Objective To explore the mediation pathway of human mesangial cell(HMC) extracellular matrix(ECM) excretion induced by ET-1.Methods Mesangial cells were cultured in DMEM medium with or without ET-1.Collagen type IV(Col IV) and fibronectin(Fn) in the supernatant were detected by enzyme-linked immumoadsorbent assay(ELISA),expression of platelet-derived growth factor-B(PDGF-B) mRNA was determined by reverse transcription polymerase chain response(RT-PCR),and expression of the protein synthesis of PDGF-B was de...

关 键 词：内皮素-1 细胞外基质 血小板源性生长因子-B 氯沙坦 

分 类 号：R587.2[医药卫生—内分泌]