nAChR_(α7)在条件免疫反应治疗小鼠支气管哮喘中的作用机制  

Mechanism of nAChRα7 in treatment of bronchial asthma by a conditioned immune response

作　　者：李化兵[1] 韩秀珍[1] 冯益真[1] 王金荣[1] 孙妍[1] 孙立峰[1] 伊迎春[1] 李志鹏[1] 

机构地区：[1]山东大学附属省立医院儿科,济南250021

出　　处：《山东大学学报（医学版）》2009年第7期16-20,共5页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助项目(30571739)

摘　　要：目的探讨nAChRα7在条件免疫反应治疗小鼠支气管哮喘中的作用机制。方法选4~6周龄的雌性BALB/C小鼠,用卵清蛋白(OVA)腹膜下注射致敏,OVA雾化吸入激发,建立支气管哮喘模型,健康对照组用PBS给予相同处理。将模型小鼠随机分为7组,每组15只。A组:CIR1组;B组:CIR2组;C组:常规治疗组;D组:常规治疗减量组;E组:单纯声音刺激组;F组:单纯糖精水刺激组;G组:空白对照组。以上7组每天给予哮喘激发1次,各治疗组在治疗间隔1 h后给予激发。健康对照组为H组,用PBS激发20周。治疗20周后,用流式细胞技术检测CD4+T细胞胞膜上烟碱型乙酰胆碱受体alpha7(nAChRα7)与胞浆内IL-4I、FN-γ表达量,分析nAChRα7与IL-4I、FN-γ的相关性。结果①A、B、C组与G组比较,CD4+T淋巴细胞表达nAChRα7I、L-4明显降低(P<0.01),IFN-γ增高(P<0.01);各因子在A、B及C组之间差异无统计学意义(P>0.05)。nAChRα7与IL-4表达呈正相关(r=0.76,P<0.01),与IFN-γ表达呈负相关(r=-0.69,P<0.01);②D、E、F、G组与H组...Objective To explore the effect of nAChRα7 on bronchial asthma by conditioned immune response(CIR) and to explore its mechanism.Methods An experimental asthma model was reproduced in healthy BALB/C mice(female,4-6weeks old)by sensitization and stimulation with OVA.All mice were randomly divided into 7 groups: CIR1(A)group,CIR2(B) group,conventional therapy(C) group,low dose conventional therapy(D) group,noise(E) group,SAC(F) group,and blank control(G) group.All the groups were stimulated with OVA at 1 hour ...

关 键 词：哮喘 条件免疫反应 nAChRα7 CD4+T淋巴细胞 小鼠 

分 类 号：R562.25[医药卫生—呼吸系统]