EFFECTS OF CHRONIC STRESS ON THE ACTIVITIES OF SOD, GSH-Px AND MDA LEVEL IN FEMALE RATS' BRAIN  

作　　者：Wang Ying(王莹) Yang Dongwei(杨东伟) Xie Wen(谢雯) Pang Wei(庞炜) Jiang Mali(蒋马莉) Han Taizhen(韩太真) 

机构地区：[1]Department of Physiology, Medical School of Xi''an Jiaotong University, Xi''an 710061,China. Department of Physiology, Medical School of Xi''an Jiaotong University, Xi''an 710061,China. Department of Physiology, Medical School of Xi''an Jiaotong University, Xi''an 710061,China. Department of Physiology, Medical School of Xi''an Jiaotong University, Xi''an 710061,China. Department of Physiology, Medical School of Xi''an Jiaotong University, Xi''an 710061,China. Department of Physiology, Medical School of Xi''an Jiaotong University, Xi''an 710061,China.

出　　处：《Academic Journal of Xi'an Jiaotong University》2002年第2期144-146,共3页西安交通大学学报（英文版）

基　　金：ThisworkwassupportedbyXi’anJiaotongUniversityasKeyProject;theNaturalScienceFoundationofShaanxiProvince ( 2 0 0 0SM39);theNationalNaturalScienceFoundationofChina(No .30 2 70 445 ) .

摘　　要：Objective To observe the effects of chronic emotional stress on the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and malonialdehyde (MDA) level in female rats’ brain. Methods The rats were randomly divided into 4 groups: normal control group (group N), emotional stress group (group E), emotional stress + pregnancy group (group E+P) and regularly drinking group (group R). Emotional stress in rats was induced by training rats with empty drinking bottles. Having been finished the stress procedure, the brain was taken out and homogenized. Then the activities of SOD, GSH-Px and MDA level were measured. Results Compared to group N, both the activities of SOD in brain tissues of group E and group E+P were significantly decreased ( P <0.05 and P <0.01, respectively) while the MDA level increased ( P <0.05). However, the extent of changes in group E+P was more obvious than that in E. GSH-Px activities in E+P and E were significantly changed. However, the GSH-Px activity in E+P was decreased ( P <0.05) while the activity in E increased ( P <0.05).Conclusion The chronic emotional stress can reduce the antioxidative system by decreasing the antioxidative enzyme activity and potentiating the lipid peroxidation in the brain. It is also suggested that the combination of emotional stress and pregnancy can augment the oxidative damage in rats’ brain.Objective To observe the effects of chronic emotional stress on the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and malonialdehyde (MDA) level in female rats' brain. Methods The rats were randomly divided into 4 groups: normal control group (group N), emotional stress group (group E), emotional stress + pregnancy group (group E+P) and regularly drinking group (group R). Emotional stress in rats was induced by training rats with empty drinking bottles. Having been finished the stress procedure, the brain was taken out and homogenized. Then the activities of SOD, GSH-Px and MDA level were measured. Results Compared to group N, both the activities of SOD in brain tissues of group E and group E+P were significantly decreased ( P <0.05 and P <0.01, respectively) while the MDA level increased ( P <0.05). However, the extent of changes in group E+P was more obvious than that in E. GSH-Px activities in E+P and E were significantly changed. However, the GSH-Px activity in E+P was decreased ( P <0.05) while the activity in E increased ( P <0.05).Conclusion The chronic emotional stress can reduce the antioxidative system by decreasing the antioxidative enzyme activity and potentiating the lipid peroxidation in the brain. It is also suggested that the combination of emotional stress and pregnancy can augment the oxidative damage in rats' brain.

关 键 词：chronic EMOTIONAL stress oxidative damage lipid PEROXIDE SOD MDA GSH-PX 

分 类 号：R338.2[医药卫生—人体生理学]