Study on Insect-resistant Transgenic Cotton Harbouring Double-gene and Its Resistance to Insect Pests  

作　　者：LI Fu-guang CUI Jin-jie LIU Chuan-liang WU Zhi-xia LI Feng-lian ZHOU Yong LI Xiu-lan GUO San-dui CUI Hong-zhi 

机构地区：[1]Cotton Research Institute,Chinese Academy of Agriculcural Sciences,Key Laboratory of Cotton Genetic Improvement,Ministry of Agriculture,Henan Anyang 455112 [2]Biotechnology Research Center,Chinese Academy of Agriculcural Sciences,Beijing 100081

出　　处：《Agricultural Sciences in China》2001年第1期70-76,共7页中国农业科学(英文版)

摘　　要：By using the method of pollen tube pathway,the synthesized GFM CryIA gene and modified CpTI gene were transfered into the elite cotton(Gossypium hirsutun L.)varieties(lines).Through the field and lab identifications,the insect-resistant transgenic plants were obtained.PCR analysis indicated that both the synthesized GFM CryIA gene and modified CpTI gene presented positive reaction.In R1 the boliworm resistance of each transformant was different,and the insect-resistance of R3 of ZGK9708 was stable.

关 键 词：Insecticidal double-gene Upland cotton Genetic transfermation BOLLWORM Insect-resistance identification 

分 类 号：S5[农业科学—作物学]