Study on the Obtaining of Transgenic Wheat with GNA Alien Gene by Biolistic Particle  

作　　者：XU Qiong-fang, LI Lian-cheng, CHEN Xiao, TIAN Fang, MA You-zhi, YE Xing-guo, ZHANG Zeng-yan, XU Hui-jun and XIN Zhi-yong( Key Laboratory of Agricultural Ministry for Crop Genetics and Breeding, Institute of Crop Breeding and Cultivation ,Chinese Academy of Agricultural Sciences, Beijing 100081 ) 

出　　处：《Agricultural Sciences in China》2002年第1期25-29,共6页中国农业科学（英文版）

摘　　要：The immature embryos of wheat plants, cv. Jing 411, 12 - 14 days after pollination, were cultured on SD2 medium for callus induction. After 10 days culture, 800 wheat calli were bombarded by biolistic particle coated with the DNA of plasmid pBI121-2 harboring both Galanthus nivalis agglutinin gene and bar gene. 67 green plants were finally regenerated from the bombardment calli on selection medium containing 4mg/L Basta. The results of bioassay by both inoculating wheat aphids onto the plants and applying Basta solution of 50 mg/L and 75 mg/L onto the wheat leaves in the field, and the molecular analysis, such as PCR and Southern blotting, indicated that 8 T2 plants contaning the target genes were obtained.: The immature embryos of wheat plants, cv. Jing 411, 12 - 14 days after pollination, were cultured on SD2 medium for callus induction. After 10 days culture, 800 wheat calli were bombarded by biolistic particle coated with the DNA of plasmid pBI121-2 harboring both Galanthus nivalis agglutinin gene and bar gene. 67 green plants were finally regenerated from the bombardment calli on selection medium containing 4mg/L Basta. The results of bioassay by both inoculating wheat aphids onto the plants and applying Basta solution of 50 mg/L and 75 mg/L onto the wheat leaves in the field, and the molecular analysis, such as PCR and Southern blotting, indicated that 8 T2 plants contaning the target genes were obtained.

关 键 词：Galanthus nivalis agglutinin bar gene Particle bombardment Transgenic wheat 

分 类 号：S512.1[农业科学—作物学]