Analysis of the Resistance Gene Analogue for Rice Cultivars in Yunnan Province  

作　　者：SUN Yan, WANG Yun-yue, HE Yue-qiu, FAN Jing-hua, CHEN Jian-bin and ZHU You-yong( Key Laboratory for Plant Pathology of Yunnan Province ,Yunnan Agricultural University, Kunming 650201 ,P.R. China  College of Agronomy and Biotechnology , Yunnan Agricultural University , Kunming 650201 , P. R . China ) 

出　　处：《Agricultural Sciences in China》2002年第5期549-554,共6页中国农业科学（英文版）

基　　金：supported by the National 863Project(AA211091);the Natural Science Foundation of China(30160048);Yunnan Agricultural University.

摘　　要：Genetic diversity of commercial and local rice cultivars in Yunnan Province was studied using the resistance gene analogue (RGA) based on resistance gene conserved sequences. The RGA analysis of 137 cultivars was conducted by PCR amplification using three primers, i.e. S1/AS3, XLRR for/XLRR rev, and Pto-kinl/Pto-kin2, respectively. The results showed that both Indica and Japonica cultivars were genetically highly diverse. All cultivars were divided into 3 lineages according to the DNA band data at 96% dissimilarity, and into 20 lineages at 60% dissimilarity. The lineages were related to their genetic background and blast disease resistance with only a few exceptions. The RGA data can be useful in rice production by mixed-planting of different cultivars in the field and breeding of resistance cultivars by selecting different parental cultivars with great genetic diversity.Genetic diversity of commercial and local rice cultivars in Yunnan Province was studied using the resistance gene analogue (RGA) based on resistance gene conserved sequences. The RGA analysis of 137 cultivars was conducted by PCR amplification using three primers, i.e. S1/AS3, XLRR for/XLRR rev, and Pto-kinl/Pto-kin2, respectively. The results showed that both Indica and Japonica cultivars were genetically highly diverse. All cultivars were divided into 3 lineages according to the DNA band data at 96% dissimilarity, and into 20 lineages at 60% dissimilarity. The lineages were related to their genetic background and blast disease resistance with only a few exceptions. The RGA data can be useful in rice production by mixed-planting of different cultivars in the field and breeding of resistance cultivars by selecting different parental cultivars with great genetic diversity.

关 键 词：RICE Resistance gene analogue Blast disease resistance 

分 类 号：S511[农业科学—作物学]