Batroxobin Against Anoxic Damage of Rat Hippocampal Neurons in Culture:Morphological Changes and Hsp70 Expression  

作　　者：刘军 匡培根 吴卫平 王福庄 丁爱石 

机构地区：[1]Neurotransmitter Research Laboratory,Chinese PLA General Hospital,Postgraduate Military Medical School,Beijing 100853 [2]Research Laboratory of Neurobiology,Military Medical Academy,Beijing 100850

出　　处：《Journal of Traditional Chinese Medicine》2001年第3期215-219,共5页中医杂志（英文版）

基　　金：军队"十五"攻关项目01MB077

摘　　要：Batroxobin,the thrombin-like enzyme,is used for therapeutic defibrination. We have found that batroxobin has good therapeutic effect in ischemic reperfusion rats and clinical practices in vivo. But we have not studied the neuroprotective effect of batroxobin on anoxic hippocampal neurons in vitro. The purpose of this study was to obtain further information on the mechanism of the batroxobin-induced neuroprotection and examine the neuroprotective effect on neurons exposed to anoxia. The effect of batroxobin on anoxic damages in cultured hippocampal neurons of neonatal rats was investigated by using morphological changes and heat shock protein 70Kd (Hsp70) immunoreactive expression as indicators. The results indicate that batroxobin, besides its defibrination, may have a direct neuroprotective effect on anoxic damage of hippocampal neurons.Batroxobin, the thrombin-like enzyme, is used for therapeutic defibrination. We have found that batroxobin has good therapeutic effect in ischemic reperfusion rats and clinical practices in vivo. But we have not studied the neuroprotective effect of batroxobin on anoxic hippocampal neurons in vitro. The purpose of this study was to obtain further information on the mechanism of the batroxobin-induced neuroprotection and examine the neuroprotective effect on neurons exposed to anoxia. The effect of batroxobin on anoxic damages in cultured hippocampal neurons of neonatal rats was investigated by using morphological changes and heat shock protein 70 Kd (Hsp70) immunoreactive expression as indicators. The results indicate that batroxobin, besides its defibrination, may have a direct neuroprotective effect on anoxic damage of hippocampal neurons.

关 键 词：ANIMALS Animals  Newborn BATROXOBIN Cell Hypoxia Cell Survival Cells  Cultured HSP70 Heat-Shock Proteins Hippocampus Neurons Neuroprotective Agents RATS Rats  Wistar 

分 类 号：R285.5[医药卫生—中药学]