维生素E对去卵巢大鼠心肌细胞凋亡的影响  

Influence of vitamin E on apoptosis of cardiac muscle cells in ovariectomized rats

作　　者：王月鹏[1] 赵行宇[2] 卢长柱[3] 姚兰春[1] 

机构地区：[1]哈尔滨医科大学生理学教研室,黑龙江哈尔滨150081 [2]吉林医药学院生理学教研室,吉林吉林132013 [3]齐齐哈尔医学院生理学教研室,黑龙江齐齐哈尔161042

出　　处：《吉林医药学院学报》2008年第2期69-71,共3页Journal of Jilin Medical University

基　　金：黑龙江省自然科学基金资助项目(D0206)

摘　　要：目的以去卵巢大鼠建立更年期动物模型,探讨维生素E(VE)对心肌细胞保护作用机制。方法正常成年大鼠和注射植物油的去卵巢大鼠为对照组;腹腔注射VE的去卵巢大鼠为实验组,分别为:5 mg/kg(小剂量组)、15 mg/kg(中剂量组)、60 mg/kg(大剂量组),连续注射9周。采用黄嘌呤氧化酶比色法、硫代巴比妥酸法测定血清中总超氧化物歧化酶(T-SOD)活力和血清丙二醛(MDA)含量水平,免疫组织化学法检测Bcl-2、Bax蛋白表达。结果各VE剂量组血清T-SOD活力均高于去卵巢大鼠对照组,MDA含量低于去卵巢大鼠对照组,且Bcl-2/Bax比值有随剂量增加呈递减趋势。结论VE可增强血清T-SOD活力,下调Bax蛋白表达,上调Bcl-2蛋白表达,抑制更年期大鼠心肌细胞凋亡。这可能是维生素E对绝经后妇女心血管保护作用的机制之一。Objective The ovariectomized rats were used as climacteric period animal model.The protective function and the mechanism of VE on the cardiac muscle cells of the ovariectomized rats were discussed.Methods The contrast group was the normal adult rats and the ovariectomized rats injected by plant oil,the experiment group was the rats extirpated ovaries who were injected VE in its′ abdomen antrum:5 mg/kg,15 mg/kg,60 mg/kg VE was injected to the experiment group for continuous 9 weeks.T-SOD,MDA,Bcl-2 and Bax were determined by the colorimetric method,the thibarbituric acid method and the immunity group chemical method.Results Compared with the negative control group,the activity of T-SOD in serum were up-regulated;the content of MDA in serum was all degraded.Bcl-2/ Bax weakened gradually along with augmentation of the dosage.Conclusion VE could improve the activity of T-SOD,reduce expression of Bax and improve expression of Bcl-2.It could also restrain the death menopause rats′ cardiac muscle cells.

关 键 词：维生素E T-SOD MDA Bcl-2 BAX 

分 类 号：R339.3[医药卫生—人体生理学]