非诺贝特和阿托伐他汀对ApoE基因敲除小鼠外周血单核细胞TLR4表达的影响  

Effects of fenofibrate and atorvastatin on Toll-like receptor(TLR) 4 expression in ApoE-deficient mice

作　　者：耿小勇[1] 韩俊燕[1] 朱柳园[1] 刘舒[1] 秦明照[1] 曾辉[1] 

机构地区：[1]100730,北京市,首都医科大学附属北京同仁医院干部医疗科 北京地坛医院传染病研究所 　 首都医科大学附属安贞医院 100730,北京市,首都医科大学附属北京同仁医院干部医疗科 北京地坛医院传染病研究所

出　　处：《中华实验和临床感染病杂志（电子版）》2008年第2期27-32,共6页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

摘　　要：目的探讨非诺贝特和阿托伐他汀对动脉粥样硬化(atherosclerosis,AS)模型小鼠单核细胞Toll样受体4(Toll-like receptor4,TLR4)表达的影响。方法apoE基因缺陷(apoE deficient,apoE-/-)小鼠被随机分为3组,分别为阿托伐他汀干预组、非诺贝特干预组、模型组,每组10只。前两组分别给予阿托伐他汀10mg.kg-1.d-1和非诺贝特100mg.kg-1.d-1,对照组给予等量蒸馏水灌胃。8周后,测定血清低密度脂蛋白(LDL)、甘油三酯(TG)、总胆固醇(TC)水平,并应用流式细胞仪检测各组小鼠外周血单核细胞表面TLR4的表达。结果各组小鼠血脂水平无统计学差异。与单纯高脂餐喂养组相比,非诺贝特干预组小鼠TLR4表达明显降低(P=0.02);阿托伐他汀干预组TLR4表达轻度升高,但无统计学意义。结论非诺贝特可降低TLR4表达,可能参与AS进程中介导的天然免疫反应。Objective To observe the effects of fenofibrate and atorvastatin on Toll-like receptor(TLR) 4 expression in ApoE-deficient mice.Methods ApoE-deficient mice were divided into 3 groups which received high-fat diet(HF) alone and were treated with fenofibrate(HF+F) or atorvastatin(HF+A) for eight weeks,respectively.Serum level of total low-density lipoprotein,cholesterol,triglyceride,total cholesterol was detected after 8 weeks,respectively.Results No significant difference of serum level of total low-density lipoprotein,cholesterol,triglyceride,total cholesterol were detected among three groups.Fenofibrate,but not atorvastatin showed significant down-regulation effect on TLR4 expression on CD14+ monocytes.Conclusions Fenofibrate could down-regulate TLR4 expression on monocytes in a lipids-lowing-independent manner,which may be involved in its anti-inflammatory mechanisms in atherosclerosis.

关 键 词：TOLL样受体 动脉粥样硬化 非诺贝特 阿托伐他汀 

分 类 号：R543.5[医药卫生—心血管疾病]