十六烷基三甲基溴化铵对DNA热稳定性的影响  

The Effects of CTAB on the Thermal Stability of DNA

作　　者：邬瑞光[1] 尉志武[1] 

机构地区：[1]清华大学化学系生命有机磷化学与化学生物学教育部重点实验室,北京100084

出　　处：《化学通报》2004年第6期439-443,共5页Chemistry

基　　金：国家自然科学基金 (2 0 1330 30 );教育部"高校青年教师奖"基金资助项目

摘　　要：研究十六烷基三甲基溴化铵 (CTAB)对 DNA分子热稳定性的影响对于认识阳离子表面活性剂与 DNA二者之间的相互作用具有重要的意义。用差示扫描量热法和变温紫外光谱法研究了CTAB对鲑鱼精 DNA及降解的鲱鱼精 DNA热稳定性的影响 ,发现 CTAB使鲑鱼精 DNA的热稳定性降低 ,但降低程度随 CTAB浓度的增大而先增大后减小 ;CTAB使鲱鱼精 DNA发生了两阶段熔解行为。Investigating the effects of cetyltrimethylammonium bromide (CTAB) on the thermal stability of DNA is of importance in understanding interactions between cationic surfactants and DNA. The modulation of CTAB on the thermal stability of salmon sperm DNA and herring sperm DNA have been investigated by employing differential scanning calorimetry and ultraviolet spectroscopy methods. It was found that the presence of CTAB decreased the thermal stability of the salmon sperm DNA, but with a gradual increase and then decrease in its effectiveness along with the increasing of CTAB concentration. Biphasic-melting behavior was observed on the herring sperm DNA when the concentration of CTAB is increased to a value about 0.23 times of phosphorous in DNA.

关 键 词：十六烷基三甲基溴化铵 DNA 热稳定性 阳离子表面活性剂 两阶段熔解行为 

分 类 号：O629[理学—有机化学]