亚蝗微粒子虫孢子体外发芽方法研究  

The method study on spores Germination of Nosema Asiaticus in vitro

作　　者：唐伟 朱荣建 安楠 

机构地区：[1]黑龙江省延寿县林业局胜利林场

出　　处：《林业勘查设计》2009年第2期101-102,共2页Forest Investigation Design

摘　　要：采用KOH法、EDTA法、H2O2法、VB12法对亚蝗微粒子虫孢子进行体外萌发诱导试验。结果表明,H2O2法效果最好,用15%H2O2溶液在30℃下处理30分钟后,其发芽率最高可达72.83%;经PH10.5-12.0的缓冲液在30℃下作用1小时后,再以PH8.0-9.0的缓冲液30℃下处理1小时,孢子的萌发率可达到50-60%;VB12法、KOH法及EDTA效果不佳,不适用于诱导亚蝗微粒子虫孢子体外萌发。The KOH measure,EDTA measure,H2O2 measure and VB12 measure were used in the experiments of spores Germination of Nosema Asiaticus in vitro. The results showed that H2O2 measure was effectivest. Under the Condition of 30℃ and solution of 15% concentration H2O2 for 30 minutes,the germination rate could reach 72.83%. Under the conditions of PH10.5-12.0 buffer for 30 minutes,and under the conditions of PH8.0-9.0 buffer and 30℃ for 1 hour,the germination rate could reach 50%-60%. The effect of the KOH measure,ED...

关 键 词：微粒子虫 体外发芽 

分 类 号：S763[农业科学—森林保护学]