The formation of improved tetraploid population of red crucian carp×common carp hybrids by androgenesis  

作　　者：DUAN Wei QIN QinBo CHEN Song LIU ShaoJun WANG Jing ZHANG Chun SUN YuanDong LIU Yun 

机构地区：[1]Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China,College of Life Sciences,Hunan Normal University,Changsha 410081,China

出　　处：《Science China(Life Sciences)》2007年第6期753-761,共9页中国科学(生命科学英文版)

基　　金：Supported by the National Natural Science Foundation of China(Grant Nos.30330480 and 30571444);the Doctoral Station of University of the Education Min-istry of China(Grant No.200405422001);the Agriculture Technological Production Foundation of the Science and Technology Ministry of China(Grant No.2005380);Important Solution Project of Hunan Province(Grant No.2006NK2008)

摘　　要：Bisexual fertile diploid androgenetic individuals(A_(0))(2n=100)were formed by androgenesis.In this way,the diploid spermatozoa from male allotetraploid hybrids(AT)(4n=200)of red crucian carp(Carassius auratus red var.)(♀)×common carp(Cyprinus carpio L.)(♂)were used to fertilize the UV-treated hap-loid eggs of goldfish(Carassius auratus),and living androgenetic diploid fish were developed.The A_(0)became sexually mature at the age of 2 years,and they fertilized with each other to form their offspring(A_(1)).In this study,we observed the chromosomal number,gonadal structure and appearance of A_(1)fish.The results are as follows:(1)In A_(1),there were 85%tetraploids(A_(1)-4n),10%triploids(A_(1)-3n)and 5%diploids(A_(1)-2n),suggesting that diploid A_(0)could produce diploid gametes.It was concluded that the formation of diploid gametes generated from diploid A_(0)was probably related to the mechanism of pre-meiotic endoreduplication.(2)Among A_(1),only A_(1)-4n possessed normal ovaries and testes.The mature males of A_(1)-4n produced white semen.Under the electron microscope,the head of diploid sperm generated by A_(1)-4n was bigger than that of haploid sperm generated by red crucian carp.In the testes of the A_(1)-4n,there were many mature normal spermatozoa with a head bearing plasma mem-brane and a tail having the typical structure of"9+2"microtubules.Between the head and the tail,there were some mitochondria.The ovaries of A_(1)-4n developed well and mainly contained II,III and IV-stage oocytes.The IV-stage oocytes were surrounded by inner and outer follicular cells.The micropyle was observed on the oolemma of follicular cells.There were abundant yolks and plenty of endoplasmic reticulum in the cytoplasm of IV-stage oocytes.Because A_(1)-2n and A_(1)-3n were distant crossing diploid hybrids and triploid hybrids respectively,they possessed abnormal gonads,and no mature semen and eggs were observed.(3)Compared with allotetraploids,the A_(1)-4n fish not only had advantages such as fast growth rate and strong re

关 键 词：allotetraploid hybrid diploid sperm haploid egg rogenesis DIPLOID rogenetic individuals 

分 类 号：N[自然科学总论]