lasI rhlI基因缺陷对铜绿假单胞菌生物膜形成影响的体外实验研究  

Effect of lasI rhlI mutant on P. aeruginosa biofilm formation in vitro

作　　者：刘晓岚[1] 宋志军[2] 吴红[3] 刘海静[1] 王立赞[1] 陈一强[4] 孔晋亮[4] 王恒壮[4] 陈海荣[4] 

机构地区：[1]济宁医学院病理生理学教研室,272013 [2]广西医科大学病理生理学教研室 [3]丹麦国家医院临床微生物科 [4]广西医科大学第一附属医院呼吸内科

出　　处：《中国医药生物技术》2007年第3期194-196,共3页Chinese Medicinal Biotechnology

摘　　要：目的研究群体感应系统在铜绿假单胞菌(PA)生物膜形成中的作用。方法采用生理盐水-吸痰管系统进行群体感应系统完整的PAO1野生型菌株与群体感应系统缺陷(lasI rhlI基因缺陷)型菌株的体外培养,分别于培养第3、7天用扫描电镜观察两种类型PAO1的生物膜形成情况。结果培养第3天PAO1野生型菌株可形成较厚、有孔状通道的成熟生物膜结构,而lasI rhlI基因缺陷型菌株仅形成明显稀薄的早期生物膜结构;培养第7天PAO1野生型菌株的生物膜增厚,lasI rhlI基因缺陷型菌株所形成的生物膜结构呈薄膜状,显著薄于野生型菌株。结论lasI rhlI基因缺陷明显影响PA的生物膜形成能力,群体感应系统在PA生物膜形成中发挥重要作用。Objective To investigate the effect of quorum-sensing system on P. aeruginosa biofilm formation in vitro. Methods Wild-type(PAO1) and mutated P. aeruginosa(lasI rhlI mutant) strains were cultured in vitro in a physiological saline-aspirating tube system. The biofilm development of the two strains were detected using electron microscopy on day 3 and day 7,respectively. Results After being cultured for 3 days,the biofilm was mature in the PAO1 P. aeruginosa,whereas it was poorly formed in lasI rhlI mutant group. After being cultured for 7 days,the PAO1 biofilm was better developed,while the lasI rhlI mutant bioflm was still a pellicular one. Conclusions lasI rhlI mutant obviously affect the formation of biofilm of PA. Quorum-sensing system plays an important role in the formation of PA biofilm.

关 键 词：生物膜 假单胞菌 铜绿 基因表达调控 细菌 

分 类 号：R378[医药卫生—病原生物学]