HPLC法测定治偏痛颗粒中芍药苷的含量  

作　　者：张良 王珊 闵盛 

机构地区：[1]四川省成都市药品检验所,610041

出　　处：《中国实用医药》2007年第18期74-75,共2页China Practical Medicine

摘　　要：目的控制治偏痛颗粒的质量。方法建立高效液相色谱法测定治偏痛颗粒中芍药苷的含量。色谱柱为岛津Shimpack CLC-ODS色谱柱(6·0mm×150mm,5μm),流动相为甲醇-0·05mol/L磷酸二氢钾溶液-醋酸-异丙醇(67:173:4:4),检测波长230nm,流速为1·0ml/min,柱温35℃。结果芍药苷进样量在0·2～2·0μg范围内线性良好,(r=0·9999),理论板数6000,方法平均回收率为100·8%。结论本法操作简单,结果可靠,可作为治偏痛颗粒的常规检验方法。Objective Studies on quality control of Zhipiantong granules.Methods The content of paeoniflorin was determined by RP-HPLC.Shimpack CLC-ODS column(6.0 mm×150 mm,5 μm)was used with a mobile phase of methanol-0.05 mol/L KH2PO4 solution-Acetic Acid-Isopropanol(67:173:4:4)and detected at 230 nm.The flow rate was 1.0ml/min.Results The Range of linearity is 0.2～2.0 μg paeoniflorin(r=0.999 9).The theoretical plate number is 6 000.The average recovery is 100.8%.Conclusion The established method is simple,reliable and suitable for the usual quality control method of Zhipiantong granules.

关 键 词：治偏痛颗粒 芍药苷 含量测定 高效液相色谱法 

分 类 号：R286.0[医药卫生—中药学]