藻黄胶囊Ⅲ号对人肾小球系膜细胞的影响  

Effect of Zaohuang capsule No.3 on the human glomerular mesangial cell

作　　者：鞠建伟[1] 李建远[2] 连培文[2] 商滨[3] 

机构地区：[1]山东省烟台毓璜顶医院,264006 [2]烟台毓璜顶医院中心实验室山东省干细胞研究中心 [3]大连医科大学

出　　处：《中国现代药物应用》2007年第1期1-3,共3页Chinese Journal of Modern Drug Application

基　　金：山东省中医药管理局立项课题(项目编号:0304JL05)

摘　　要：目的研究藻黄胶囊Ⅲ号(ZHC3)对人肾小球系膜细胞(GMC)增殖的影响,其对GMC分泌纤维连接蛋白(FN)、白细胞介素-6(IL-6)、转化生长因子-β1(TGF-β1)的影响,以期从分子细胞水平探讨ZHC3防治慢性肾功能衰竭(CRF)的机理。方法ZHC3孵育体外培养的GMC,采用MTT法检测GMC增殖,ELISA法检测FN、IL-6、TGF-β1的分泌水平。结果ZHC3可显著抑制GMC的增殖(P<0.01),显著减少FN、IL-6、TGF-β1的分泌(P<0.01)。结论ZHC3可减少肾小球内细胞外基质堆积,延缓肾小球硬化,这可能是ZHC3防治CRF的机理。Objective To explore the mechanism that Zaohuang capsule No.3(ZHC3) cures chronic renal function failure,we investigated the effect of ZHC3 on the human glomerular mesangial cell (GMC) proliferation,fibronectin(FN),interleukin-6(IL-6) and transforming growth factor β1(TGF-β1) secretion.Methods In the present of ZHC3,GMC proliferation was detected by MTT,the levels of FN,IL-6,TGF-β1 were by ELISA.Results ZHC3 inhibited significantly GMC proliferation(P<0.01) and decreased FN,IL-6,TGF-β1 release.Conclusion ZHC3 was shown to inhibited GMC proliferation and decreased FN,IL-6,TGF-β1 release,so can reduce the cumulation of extracelluar matrix,delay glomerular sclerosis.This may be the mechanism that ZHC3 cures chronic renal function failure.

关 键 词：藻黄胶囊Ⅲ号 肾小球系膜细胞 纤维连接蛋白 白细胞介素-6 转化生长因子Β1 

分 类 号：R285[医药卫生—中药学]