柱层析-比色法测定大鼠口服低分子肝素的绝对生物利用度  

Determination of absolute bioavailability of rats after oral low molecular weight heparin by column chromatogram-colorimetry

作　　者：杨人泽[1] 魏桂林[1] 廖志宪[1] 鲁陈[1] 曾靖[2] 

机构地区：[1]赣州市赣南医学院第一附属医院,341000 [2]赣南医学院第三附属医院

出　　处：《中国现代药物应用》2007年第8期1-2,共2页Chinese Journal of Modern Drug Application

基　　金：江西省卫生厅2006年立项课题(项目编号:20063165)

摘　　要：目的通过实验明确大鼠口服LMWH的绝对生物利用度,为研制LMWH口服制剂提供依据。方法从大鼠的肝静脉采血,应用柱层析-比色法测定血中LMWH的含量,求得LMWH的绝对生物利用度。结果测定方法的标准曲线方程是A=0.515 8 C-0.0006(r=0.999 8,n=6),回收率是97.14%(RSD=1.03%,n=5),日内、日间标准差分别是6.8%、6.4%。求得绝对生物利用度89.61%。结论本实验证实,研制LMWH口服制剂是可行的。Objective To provide significant basis for development of LMWH oral preparation though determination of absolute bioavailability of rats after oral LMWH.Methods Blood was sampled through hepatic vein in rats,and the absolute bioavailability of LMWH can be calculated based on its concentration in blood, which was determinate by column chromatogram-colorimetry.Results The equation of calibration curve was A =0.5158C-0.0006 with a correlation coefficient of 0.9998.The recovery was 97.14% and RSD was 1.03%(n =5).Within-day and between-day RSDs were 6.8% and 6.4%,respectively.The absolute bioavailability was 89.61%.Conclusion The result shows that development of LMWH oral preparation is feasible.

关 键 词：大鼠 LMWH口服制剂 柱层析-比色测定法 绝对生物利用度 

分 类 号：R96[医药卫生—药理学]