机构地区:[1]State Key Laboratory for Molecular Virology and Genetic Engineering,Beijing 100176,China [2]School of Medicine,Xi’an Jiaotong University,Xi’an 710061,China [3]School of Pharmaceutical Engineering,Shengyang Pharmaceutical University,Shengyang 110016,China
出 处:《Science China(Life Sciences)》2006年第1期46-52,共7页中国科学(生命科学英文版)
摘 要:Comparative Genomic Hybridization (CGH) microarray analysis was used tocompare the genomic compositions of all eighteen Shigella boydii serotype representative strains.The results indicated the genomic ''backbone'' of this subgroup contained 2552 ORFs homologous tononpatho-genic E. coli K12. Compared with the genome of K12199 ORFs were found to be absent in allS. boydii serotype representatives, including mainly outer membrane protein genes and O-antigenbiosynthesis genes. Yet the specific ORFs of S. boydii subgroup contained basically bacteriophagegenes and the function unknown (FUN) genes. Some iron metabolism, transport and type Ⅱ secretionsystem related genes were found in most representative strains. According to the CGH phylogeneticanalysis, the eighteen S. boydii serotype representatives were divided into four groups, in whichserotype C13 strain was remarkably distinguished from the other serotype strains. This groupingresult corresponded to the distribution of some metabolism related genes. Furthermore, the analysisof genome backbone genes, specific genes, and the phylogenetic trees allowed us to discover theevolution laws of S. boydii and to find out important clues to pathogenesis research, vaccinationand the therapeutic medicine development.Comparative Genomic Hybridization (CGH) microarray analysis was used to compare the genomic compositions of all eighteen Shigella boydii serotype representative strains. The results indicated the genomic “backbone” of this subgroup contained 2552 ORFs homologous to nonpatho-genic E. coli K12. Compared with the genome of K12199 ORFs were found to be absent in all S. boydii serotype representatives, including mainly outer membrane protein genes and O-antigen bio-synthesis genes. Yet the specific ORFs of S. boydii subgroup contained basically bacteriophage genes and the function unknown (FUN) genes. Some iron metabolism, transport and type II secretion system related genes were found in most representative strains. According to the CGH phylogenetic analysis, the eighteen S. boydii serotype representatives were divided into four groups, in which se-rotype C13 strain was remarkably distinguished from the other serotype strains. This grouping result corresponded to the distribution of some metabolism related genes. Furthermore, the analysis of genome backbone genes, specific genes, and the phylogenetic trees allowed us to discover the evo-lution laws of S. boydii and to find out important clues to pathogenesis research, vaccination and the therapeutic medicine development.
关 键 词:S.boydii comparative genomic hybridization(CGH) strain-specific ORFs EVOLUTION
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