A laser light scattering study of the interaction between human serum albumin and ampicillin sodium  

作　　者：WANG Weiping TANG Jianghong PENG Xuhong HU Zhide CHEN Xingguo 

机构地区：[1]Department of Chemistry, Lanzhou University, Lanzhou 730000, China Department of Chemistry, Lanzhou University, Lanzhou 730000, China Department of Chemistry, Lanzhou University, Lanzhou 730000, China Department of Chemistry, Lanzhou University, Lanzhou 730000, China Department of Chemistry, Lanzhou University, Lanzhou 730000, China

出　　处：《Science China Chemistry》2006年第4期332-337,共6页中国科学（化学英文版）

基　　金：This work was supported by the National Natural Science Foundation of China (Grant No.20275014).

摘　　要：The complexes formed by the interaction of human serum albumin and ampicillin sodium in aqueous solutions were investigated at 25 ± 0.1℃, ionic strength I = 0.085 mol·kg-1, pH 4.9, 5.8 and 7.4. The results of static light scattering have suggested that at pH 7.4, 5.8, 4.9, the molecular weight of the protein/drug complexes is 210,000 g·mol-1, 418,000 g·mol-1, 448,000 g·mol-1, re- spectively. The z-average root-mean-square radius of gyration and the second virial coefficients de- crease with pH decreasing. Dynamic light scattering provides information on diffusion coefficient and particle distributions of protein/drug complexes under different conditions, which suggests a broad hydrodynamic diameter range of scatters. The diffusion coefficients of the systems change with am- picillin sodium concentration and pH changing.The complexes formed by the interaction of human serum albumin and ampicillinsodium in aqueous solutions were investigated at 25 ± 0.1℃, ionic strength I = 0.085mol·kg^(-1), pH 4.9, 5.8 and 7.4. The results of static light scattering have suggested that at pH7.4, 5.8, 4.9, the molecular weight of the protein/drug complexes is 210,000 g·mol^(-1), 418,000g·mol^(-1), 448,000 g·mol^(-1), respectively. The z-average root-mean-square radius of gyrationand the second virial coefficients decrease with pH decreasing. Dynamic light scattering providesinformation on diffusion coefficient and particle distributions of protein/drug complexes underdifferent conditions, which suggests a broad hydrodynamic diameter range of scatters. The diffusioncoefficients of the systems change with ampicillin sodium concentration and pH changing.

关 键 词：human serum ALBUMIN (HSA)  AMPICILLIN sodium (AMP)  dynamic LIGHT SCATTERING (DLS)  static LIGHT SCATTERING (SLS). 

分 类 号：N[自然科学总论]