Preparation of PLL-PEG-PLL and its application to DNA encapsulation  

作　　者：YANG Zigang1, ZHOU Fen1, YUAN Jianjun1, MA Lixin2, ZHAI Chao2 & CHENG Shiyuan1 1. School of Chemistry and Materials Science, Hubei University, Wuhan 430062, China 2. School of Life Science, Hubei University, Wuhan 430062, China 

出　　处：《Science China Chemistry》2006年第4期357-362,共6页中国科学（化学英文版）

摘　　要：PLL-PEG-PLL was synthesized by deprotection of the amino group of PLL(Z)-PEG-PLL(Z). The properties of the polyion complex (PIC) micelles comprising of PLL-PEG-PLL and DNA were investigated. The results showed that the core of PIC was formed by PLL and DNA through electrostatic interactions, and PEG was shell of PIC, so PIC micelles in aque- ous medium were homogenous with no precipitation even under the neutral condition, and PIC had the characteristic of stability, which were very important in gene therapy. At the same time, the results showed PLL-PEG-PLL could condense DNA, longer PLL blocks were generally more effective at condensation, and the nuclease resistance of DNA was increased remarkably. PIC was spherical with diameter of 10―35 nm, which could be easily taken up by cells. Transfection experiments in vitro showed that the DNA encapsulated could be expressed in the cell line SF9.PLL-PEG-PLL was synthesized by deprotection of the amino group ofPLL(Z)-PEG-PLL(Z). The properties of the polyion complex (PIC) micelles comprising of PLL-PEG-PLLand DNA were investigated. The results showed that the core of PIC was formed by PLL and DNA throughelectrostatic interactions, and PEG was shell of PIC, so PIC micelles in aqueous medium werehomogenous with no precipitation even under the neutral condition, and PIC had the characteristic ofstability, which were very important in gene therapy. At the same time, the results showedPLL-PEG-PLL could condense DNA, longer PLL blocks were generally more effective at condensation, andthe nuclease resistance of DNA was increased remarkably. PIC was spherical with diameter of 10—35nm, which could be easily taken up by cells. Transfection experiments in vitro showed that the DNAencapsulated could be expressed in the cell line SF9.

关 键 词：poly(L-Lysine)  poly(ethylene glycol)  block copolymer  DNA  polyion complex micelles (PIC). 

分 类 号：Q523[生物学—生物化学]