8-羟基喹哪啶固相萃取光度法测定生物样品中的铁  

Determination of Iron in Biological Sample by Solid Phase Extraction Spectrophotometry with 8-Hydroxyquinadine

作　　者：台希[1] 

机构地区：[1]玉溪师范学院化学与环境科学系,云南玉溪653100

出　　处：《玉溪师范学院学报》2006年第3期18-20,共3页Journal of Yuxi Normal University

摘　　要：研究了8-羟基喹哪啶与铁的显色反应,在pH为8.0的氯化氨-氨水缓冲介质中,在乳化剂-OP存在下,8-羟基喹哪啶与铁反应生成3∶1的稳定的蓝紫色络合物.该络合物可被W aters Plus-C18固相萃取小柱萃取富集,富集的络合物用乙醇为洗脱剂洗脱后用光度法测定.在乙醇介质中,络合物最大吸收波长λmax=595 nm,ε=7.82×103L.mol-1.cm-1.铁含量在0.1~5.0μg/mL内符合比尔定律,方法用于生物样品中痕量铁的测定,结果令人满意.The color reaction of iron(III) with 8hydroxyquinadine was studied.In the NH_4Cl-NH_3.H_2O buffer solution with a pH value of 8.0,and emulsifier-OP as medium,8-hydroxyquinadine reacts with iron(III) to form a stable 3 ∶1 complex.This complex can be extracted by Waters Plus-C_(18) cartridge.After the enrichment is finished,the retained chelate can be eluted by ethanol.In ethanol medium,the molar absorptivity of the chelate is 7.82×10~3 L·mol^(-1)·cm^(-1) at 595 nm.Beer's law is obeyed in the range of 1.0～5.0 μg/mL.This method can be applied to the determination of iron in biological sample with satisfactory results.

关 键 词：8-羟基喹哪啶 铁 测定 固相萃取光度法 生物样品 

分 类 号：O657.32[理学—分析化学]