板蓝根多糖对鹅胚成纤维细胞增殖的影响  

Effects of isatis root polysaccharide on proliferation of goose embryo fibroblast

作　　者：李青[1] 许小琴[1] 王志刚[1] 王彬[1] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2009年第3期61-63,共3页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(30471272);扬州大学自然科学基金资助项目(2007CXJ024)

摘　　要：将6种不同浓度的板蓝根多糖(IRPS)分别加入经培养24 h形成单层的鹅胚成纤维细胞(GEF)中,加药培养12、24、36、48、60和72 h后,分别用四唑盐法研究IRPS对GEF增殖的影响。结果表明:IRPS含量为100~1 000μg.mL-1能够显著促进GEF增殖,且呈一定的量效和时效关系。Six different concentrations of isatis root polysaccharide(IRPS) were respectively added to monolayer of goose embryo fibroblast(GEF) which had been cultured for 24 h.After 12,24,36,48,60 and 72 h medication,their effects on GEF proliferation were respectively assayed by MTT methods.The results showed that IRPS with the concentrations of 100-1 000 μg·mL-1 obviously promoted the cell proliferation,which had relations to dose-and time-dependence effect.

关 键 词：板蓝根多糖 鹅胚成纤维细胞 细胞增殖 

分 类 号：S835[农业科学—畜牧学]