超抗原SEB诱导的免疫耐受与细胞因子无关  

The effector cells activated by superantigen SEB leading to tolerance rather than cytokines

作　　者：陈钰[1] 郭业磊[1] 钟江[2] 陈中才[2] 

机构地区：[1]解放军总医院免疫学教研室 [2]复旦大学生命科学院微生物与微生物工程系,上海200433

出　　处：《免疫学杂志》2009年第4期393-396,共4页Immunological Journal

基　　金：国家自然科学基金资助项目(30671981和30571650)

摘　　要：目的研究超抗原金黄色葡萄球菌肠毒素B(staphylococcal enterotoxin B,SEB)诱导的耐受效应和耐受调节机制。方法收集SEB活化10天的细胞,充分洗涤后做为效应细胞,分别与刀豆蛋白(ConA)、脂多糖(LPS)和白介素-2(IL-2)共同培养,用MTT方法测定细胞的耐受性应答反应。正常淋巴细胞在与ConA、LPS、IL-2共同培养的同时添加效应细胞,用MTT方法测定效应细胞的抑制性应答反应功能。流式细胞技术解析耐受性效应细胞类型。结果效应细胞对ConA、LPS和IL-2的应答反应能力明显降低(P<0.01,n=3),但保持对ConA的应答反应能力。效应细胞抑制正常淋巴细胞与ConA、LPS和IL-2的应答反应(P<0.01,n=3),尤其抑制ConA和IL-2诱导的细胞增殖。SEB活化的效应细胞中CD8+NK1.1+、TcRVβ8+NK1.1+和CD4+NK1.1+NKT细胞以及TcRVβ8+T、CD8+T细胞数量明显增加(P<0.01和P<0.05,n=4)。结论超抗原SEB诱导的耐受性应答反应是效应细胞的直接作用,与细胞因子无关;这些效应细胞能抑制T淋巴细胞增殖,并保存识别ConA的受体功能。Objective To investigate the mechanisms of the immune tolerance induced by superantigen staphylococcal enterotoxin B(SEB).Methods Splenic lymphocytes from C57BL/J mice were cultured with SEB for 10 days,and then washed and collected as effector cells.ConA,LPS,and IL-2 were cocultured with the effector cells for measuring the responsivity of the effector cells by MTT method.The effector cells were also cultured with normal lymphocytes and above mitogens or cytokines.Inhibitory effects on immune regulation of...

关 键 词：超抗原SEB 免疫耐受 细胞因子 NKT细胞 

分 类 号：R392[医药卫生—免疫学]