青蒿中6种成分同时定量的高效液相色谱法研究  

Simultaneous Quantitative Analysis of Six Ingredients in Artemisia Annua L. by HPLC

作　　者：薛洪宝[1] 焦艳娜[1] 庞国伟[1] 刘海峰[1] 唐静[1] 李晖[1] 

机构地区：[1]四川大学化学工程学院,四川成都610065

出　　处：《四川大学学报（工程科学版）》2009年第4期119-124,共6页Journal of Sichuan University (Engineering Science Edition)

基　　金：国家自然科学基金资助项目(29877018)

摘　　要：利用高效液相色谱建立了中药青蒿中青蒿素、芦丁、异槲皮苷、水杨酸、槲皮苷、槲皮素6种成分同时定量的分析方法,其中线性范围宽、相对标准偏差低、精密度高、重现性好。色谱最佳条件是:Tiahhe Kromasil C18 100 A(5μm,250 mm×4.6 mm)反相色谱柱;紫外检测波长:279 nm;流动相:V(甲醇)∶V(水)=50∶50(含0.02%三氟乙酸);流速:0.5 ml/min;柱温:30℃。同时与蒸发光散射检测器相比较,除青蒿素外其它成分检测灵敏度高。A general method to separate and quantify simultaneously 6 active ingredients(arteannuin,rutin,isoquercitrin,salicylic acid,quercitrin,quercetin) in traditional Chinese medicine Artemisia annua L.was established by high performance liquid chromatography(HPLC).This method had a wide linear range,low relative standard deviation,high precision and perfect reproducibility.Optimized apparatus condition was explored as followings An Tiahhe Kromasil C18 100 A(5 μm,250 mm×4.6 mm) reversed-phase column was applied u...

关 键 词：青蒿素 同时定量分析 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]