犬腺病毒DNA疫苗的免疫实验研究  

An empirical study of canine adenovirus DNA vaccine

作　　者：张霞[1,2] 郑龙[2] 王俊霞[2] 张焕玲[2] 尤红煜[2] 刘福英[2] 

机构地区：[1]山东潍坊医学院附属医院检验科 [2]河北医科大学实验动物学部分子生物学研究室

出　　处：《免疫学杂志》2009年第5期550-553,共4页Immunological Journal

摘　　要：目的研究犬腺病毒DNA疫苗pVAX1-CpG-Loop刺激机体产生免疫应答的效果。方法采用2种不同的免疫途径和免疫方案免疫Balb/c小鼠;免疫后每周断尾采血,分离血清测定血清IgG抗体效价;采用MTT和CCK-8方法检测免疫小鼠的T淋巴细胞增殖活性,EIA试剂盒测定IFN-γ的浓度。结果所有接种DNA疫苗的小鼠均产生了针对抗原病毒的特异性IgG抗体。细胞学试验提示构建的犬腺病毒DNA疫苗也可诱导小鼠产生细胞性免疫应答,重组质粒-蛋白联合的免疫方案在刺激机体细胞免疫应答方面优于单一重组质粒。结论以上结果提示犬腺病毒DNA疫苗pVAX1-CpG-Loop既能诱导小鼠产生特异性的体液免疫应答,也诱导小鼠产生了细胞免疫应答,对于机体具有一定的免疫保护效果。Objective To investigate the immune efficacy of canine adenovirus DNA vaccine pVAX1-CpG-Loop.Methods Balb/c mice were immunized with constructed DNA vaccine pVAX1-CpG-Loop in two different immune routes and strategy.Sera from all animals were collected from tail weekly to detect IgG antibody;MTT and CCK-8 cell counting kit were used to evaluate the activity of T lymphocytes proliferation;EIA kit was used to detect the levels of IFN-γ in supernatants of splenocyte.Results Specific IgG antibody against CAV-1 ...

关 键 词：犬腺病毒I型 DNA疫苗 重组质粒 免疫效果 

分 类 号：R392-33[医药卫生—免疫学]