NF-κB不参与ONO-AE-248诱发的中性粒细胞非凋亡、非坏死性死亡  

NF-κB is not involved in ONO-AE-248-induced non-apoptotic or non-necrotic neutrophil death

作　　者：李国利[1] 胡艳玲[1] 刘佳佳[1,2] 

机构地区：[1]泸州医学院免疫学实验室 [2]泸州医学院分子生物学实验室

出　　处：《免疫学杂志》2009年第5期515-518,523,共5页Immunological Journal

基　　金：国家自然科学基金资助项目(30371305)

摘　　要：目的研究核转录因子NF-κB(Nuclear factor kappa B)在ONO-AE-248诱发中性粒细胞非凋亡、非坏死性死亡中的作用,探讨这种细胞死亡的分子机制。方法Ficoll法分离健康志愿者外周静脉血中性粒细胞,与ONO-AE-248共同培养,通过Western blotting检测IκBα的蛋白表达水平,反映NF-κB的活化状态,并应用RT-PCR检测NF-κB调节的基因XIAP、DAD1和FLIP的mRNA表达水平。结果ONO-AE-248不能促进中性粒细胞内IκBα蛋白降解,显示它并不诱导NF-κB活化。而且,ONO-AE-248对NF-κB调节的基因XIAP、DAD1、FLIP的mRNA表达也无明显影响(P>0.05)。结论ONO-AE-248诱发的中性粒细胞非凋亡、非坏死性死亡不依赖NF-κB信号转导通路,其介导中性粒细胞死亡的信号转导可能是不同于凋亡的独特转导途径。Objective To investigate the role of nuclear factor kappa B(NF-κB)in the regulation of ONO-AE-248-induced neutrophil death.Methods Heparinized peripheral blood was obtained from healthy volunteers.Neutrophils were isolated by density gradient centrifugation with Ficoll,then cultured in the presence or absence of ONO-AE-248(5 × 10-5 mol/L)for the indicated time periods.NF-κB activation was examined by Western blotting analysis.Expression levels of NF-κB-regulated genes,including X-linked inhibitory apoptosis...

关 键 词：NF-ΚB 中性粒细胞 ONO-AE-248 非凋亡非坏死性细胞死亡 信号转导 

分 类 号：R392.12[医药卫生—免疫学]