金莲花黄酮对K562、HeLa、Ec-109、NCI-H446细胞增殖的影响  

Effects of Trollius flavonoids on proliferation of K562,HeLa,Ec-109,and NCI-H446 tumor cells

作　　者：孙黎[1] 程建贞[2] 罗强[1] 张秀昌[2] 白雪梅[1] 安芳[1] 

机构地区：[1]河北北方学院实验中心,张家口075029 [2]河北北方学院医学技术学院,张家口075029

出　　处：《郑州大学学报（医学版）》2009年第5期981-983,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：河北省教育厅基金资助项目2007303

摘　　要：目的:观察金莲花黄酮对K562、HeLa、Ec-109、NCI-H446肿瘤细胞增殖的影响。方法:①采用CCK-8法检测0.793、1.586、3.172、6.344、9.516、12.688g/L金莲花黄酮对K562、HeLa、Ec-109、NCI-H446肿瘤细胞株增殖的影响。②采用流式细胞术观察0.793、1.586、3.172g/L金莲花黄酮对K562细胞周期的影响。结果:①金莲花黄酮在0.793～12.688g/L范围内对体外培养的K562、HeLa、Ec-109、NCI-H446肿瘤细胞生长均有明显的抑制作用,且抑制作用呈剂量依赖性(F=6.72,9.81,8.54,4.83,P均<0.05)。②金莲花黄酮可使K562G0/G1期细胞增多,S期细胞减少(F=12.56,20.86,P均<0.05)。结论:金莲花黄酮对体外培养的K562、HeLa、Ec-109、NCI-H446肿瘤细胞株的增殖均有很强的抑制作用;金莲花黄酮可使体外培养的K562细胞周期阻滞在G0/G1期,可能是其抑制K562肿瘤细胞增殖的机制。Aim:To observe the effects of Trollius flavonoids on proliferation of K562,HeLa,Ec-109,and NCI-H446 tumor cells.Methods:By the method of CCK-8,the effects of Trollius flavonoids with the concentration of 0.793,1.586,3.172,6.344,9.516,12.688 g/L on proliferation of K562,HeLa,Ec-109,and NCI-H446 tumor cells were detected. By flow cytometry,the effects of Trollius flavonoids with the concentration of 0.793,1.586 and 3.172 g/L on K562 cell cycle were observed.Results:Trollius flavonoids at 0.793~12.688 g/L had ...

关 键 词：金莲花黄酮 K562 HELA Ec-109 NCI-H446 增殖 

分 类 号：R285[医药卫生—中药学]