IL-18通过JNK途径诱导肾小管上皮细胞转分化  

Interleukin-18-induced transdifferentiation in renal proximal tubular cells is mediated by activation of JNK pathway

作　　者：姚翠微[1] 张军峰[1] 刘华锋[1] 梁东[1] 唐德燊[1] 陈孝文[1] 

机构地区：[1]广东医学院附属医院肾病研究所

出　　处：《免疫学杂志》2009年第6期629-631,637,共4页Immunological Journal

基　　金：广东省自然科学基金(5011589)

摘　　要：目的探讨白细胞介素18(IL-18)是否通过JNK细胞内信号转导途径诱导近端肾小管上皮细胞转分化。方法应用体外细胞培养技术培养人近端肾小管上皮细胞株(HK-2细胞),分别予不同浓度的JNK通路特异性阻断剂SP600125预孵育HK-2细胞30min后,加入IL-18共培养,于24h、48h和72h3个时点分别收集细胞。应用逆转录多聚酶链反应(RT-PCR)检测α-SMA mRNA的表达水平,应用酶联免疫吸附法(ELISA方法)测定细胞α-SMA的表达水平。结果SP600125可抑制IL-18诱导的HK-2细胞α-SMA基因和蛋白的表达,且呈一定程度的剂量依赖趋势。结论JNK通路可能是调控IL-18所诱导肾小管上皮细胞转分化的信号通路之一。Objective To explore the effect of JNK signaling pathway on interleukin-18 (IL-18)-induced transdifferentiation in renal proximal tubular cells. Methods Human proximal tubular epithelial cell line (HK-2 cells) was cultured in vitro. After preincubation with SP600125 (0,5,10,20 μmol/L) for 30 minutes,cells were exposed to IL-18 (100 ng/mL) for 24,48,and 72 h,respectively. The expressions of α-SMA in cultured HK-2 cells were assessed by RT-PCR and ELISA. Results SP600125 could obviously inhibit the IL-18-indu...

关 键 词：肾间质纤维化 肾小管上皮细胞 转分化 白细胞介素18 JNK通路 

分 类 号：R692[医药卫生—泌尿科学]