葛根素对牛主动脉内皮细胞增殖的影响  

Effects of puerarin on proliferation of bovine artery endothelial cells

作　　者：赵雪艳[1] 李凌[2] 许予明[3] 

机构地区：[1]河南大学淮河医院神经内科,开封475000 [2]郑州大学第一附属医院心内科,郑州450052 [3]郑州大学第一附属医院神经内科,郑州450052

出　　处：《郑州大学学报（医学版）》2009年第6期1250-1253,共4页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:观察葛根素(Pue)对牛主动脉内皮细胞(BAECs)增殖的影响。方法:取BAECs细胞,分为空白对照组(C组):常规培养24h;溶媒组(S组):加PBS后培养24h;葛根素(Pue)低、中及高剂量组:分别加入终质量浓度为0.5g/L(P1组)、1.0g/L(P2组)及1.5g/L(P3组)的Pue,再培养24h。另取细胞,同上分组,均于加药前2h加入L-单甲基精氨酸(L-NMMA),加药后再培养24h。采用MTT法检测细胞增殖情况,流式细胞仪检测细胞周期,WesternBlot检测细胞内皮型一氧化氮合酶(eNOS)的表达。结果:Pue可促进BAECs的增殖,升高S+G2/M期的细胞比例,促进BAECseNOS蛋白的表达(F=277.27,570.65,1051.99,P<0.001),而L-NMMA可抑制上述效应。结论:Pue可促进BAECs进入分裂期从而促进增殖,其机制可能与增加eNOS的表达从而上调NO水平有关。Aim:To observe the effects of puerarin(Pue)on the proliferation of bovine artery endothelial cells(BAECs).Methods:Ⅰ:BAECs were cultured and allocated into group C,group S and low,middle and high-dose Pue groups(group P1,P2,and P3).Group C was not treated;group S and group P1,P2 and P3 were given PBS,low,middle and high-dose Pue and then cultured for 24 h.Ⅱ:BAECs were allocated into 5 groups as above but added L-NMMA 2 h before administration.MTT and flow cytometry were used to detect cell proliferation and ...

关 键 词：葛根素 牛 内皮细胞 细胞增殖 

分 类 号：R285.5[医药卫生—中药学]