连翘抗病毒有效部位(LC-4)体外抗呼吸道合胞病毒作用的研究  

Effect of an Active Component from Forsythia suspensa (Thunb.) Vahl against Respiratory Syncytial Virus in Vitro

作　　者：陈杨[1] 李鑫[1] 周婧瑜[1] 杨庆伟[1] 李洪源[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院流行病教研室,哈尔滨150081

出　　处：《卫生研究》2009年第6期733-735,共3页Journal of Hygiene Research

摘　　要：目的研究连翘抗病毒有效部位(LC-4)的提取分离及其体外抗呼吸道合胞病毒(RSV)的作用。方法将连翘水提液经水提醇沉法、大孔吸附树脂柱层析法,洗脱剂洗脱分离得到连翘抗病毒有效部位LC-4,采用细胞病变(CPE)观察法和中性红实验法,以利巴韦林为阳性对照药,测定LC-4在最大无毒浓度下于Hela细胞中对RSV的预防作用及治疗作用。结果LC-4的半数中毒浓度(TC50)为25.41μg/ml,半数有效浓度(EC50)为2.11μg/ml,治疗指数(TI)为12.04;LC-4在RSV感染Hela细胞2、4、6、8h后给药,与病毒对照组相比CPE程度差异有显著性(P<0.05);且提前2h给药在LC-4浓度为31.25～3.91μg/ml之间时,与病毒对照相比CPE差异有显著性(P<0.05)。结论LC-4在体外对RSV复制有明显的预防作用及治疗作用。Objective To study the extraction of an active component LC-4 from Forsythia suspensa(Thunb.)Vahl and anti-respiratory syncytial virus(RSV)effect in vitro.Methods The extration from boiling water of Forsythia suspensa were extracted by hot water extraction and were precipitated by ethanol and were elicited by macroporous adsorptive resins column chromatography.Then the antiviral active component LC-4 was separated from the production by eluting.The cytotoxicities and antiviral effect of LC-4 were tested by ...

关 键 词：连翘 呼吸道合胞病毒 抗病毒作用 中药 

分 类 号：R285[医药卫生—中药学]