柑橘内生细菌YS45的鉴定、抗菌物质分析及其对油菜菌核病的防治作用  

Characterization of endophytic bacterial strain YS45 from the citrus xylem and its biocontrol activity against Sclerotinia stem rot of rapeseed

作　　者：张翼[1] 白晨[1] 冉国华[2] 张志元[2] 陈耀辉[1] 吴刚[1] 

机构地区：[1]复旦大学生命科学学院生物化学系,上海200433 [2]长沙学院生物工程与环境科学系,长沙410003

出　　处：《植物病理学报》2009年第6期638-645,共8页Acta Phytopathologica Sinica

基　　金：教育部重点科技项目(01087);长沙市科技项目(K051085-22)

摘　　要：从柑橘枝条中分离到26株对油菜菌核病菌(Sclerotinia sclerotiorum)具有拮抗作用的内生细菌,其中YS45菌株的拮抗活性最强。16S rRNA基因序列分析及形态学和生理生化鉴定结果表明YS45菌株为枯草芽孢杆菌(Bacillus subtilis)。高效液相色谱及质谱分析结果显示其主要的抑菌活性物质为一组fengycin同系物,包括fengycins A、fengycins B和一种稀少fengycin类型化合物。油菜离体叶片接种试验中,YS45菌株发酵液对油菜菌核病的防效在70%以上,与五氯硝基苯相当;田间小区接种试验表明,YS45菌株发酵液对油菜菌核病的防效也在50%以上。Twenty six bacterial strains with antagonistic activities against Sclerotinia sclerotiorum were iso-lated from the branch xylem of healthy citrus plants.Among them,strain YS45,which showed the strongest activity,was identified as Bacillus subtilis based on the analysis of 16S rDNA gene sequence,the morphologic characteristics and physiologic-biochemical characteristics.High Performance Liquid Chromatography(HPLC) and Mass Spectrometry(MS) analysis indicated that the main antifungal compounds from broth of s...

关 键 词：内生细菌 柑橘 抑菌物质 油菜菌核病 生物防治 

分 类 号：S476.8[农业科学—农业昆虫与害虫防治]