蚕豆染色病毒CPS基因的序列测定及RT-PCR检测方法的建立  

Sequence determination of small coat protein gene of Broad bean stain virus and the development of RT-PCR detection method

作　　者：陈红运[1] 陈青[1] 黄峰[1] 赵文军[2] 廖富荣[1] 陈洪俊[2] 朱水芳[2] 

机构地区：[1]厦门出入境检验检疫局,厦门361026 [2]中国检验检疫科学研究院,北京100029

出　　处：《植物病理学报》2009年第6期646-649,共4页Acta Phytopathologica Sinica

基　　金：国家科技支撑计划项目(2006BAD08A132006BAK04A02);国家质检总局资助项目(2007IK253)

摘　　要：The nucleotide sequence of small coat protein(CPS) gene of Broad bean stain virus(BBSV) was determined and compared with other comoviruses.The CPS gene of BBSV consisted of 687 nucleotides and encodes a putative protein of 228 amino acid residues.The CPS sequence of BBSV and those of other comoviruses shared identities of 36.5%-58.9% and 35.2%-70.3% at the nucleotide and amino acid levels,respectively.The RT-PCR method specific for BBSV detection was developed based on the determined CPS sequence.The RT-PCR assay presented here allows,for the first time,rapid and specific detection of BBSV.The nucleotide sequence of small coat protein(CPS) gene of Broad bean stain virus(BBSV) was determined and compared with other comoviruses.The CPS gene of BBSV consisted of 687 nucleotides and encodes a putative protein of 228 amino acid residues.The CPS sequence of BBSV and those of other comoviruses shared identities of 36.5%-58.9% and 35.2%-70.3% at the nucleotide and amino acid levels,respectively.The RT-PCR method specific for BBSV detection was developed based on the determined CPS sequence.The RT-PCR...

关 键 词：Broad bean stain virus small coat protein detection 

分 类 号：S432.41[农业科学—植物病理学]