水稻白叶枯病菌在寄主体内外不同生长条件下致病基因差异表达的分析  

Differential expression of pathogenicity genes of Xanthomonas oryzae pv.oryzae and deletion mutants ΔgacAxoo and ΔfleQxoo in vitro and in planta revealed by DNA microarray analysis

作　　者：高世强[1] 张新建[1] 吴茂森[1] 何晨阳[1] 

机构地区：[1]中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京100193

出　　处：《植物病理学报》2009年第6期653-658,共6页Acta Phytopathologica Sinica

基　　金：国家自然科学基金资助项目(30671353);国家农业行业科研专项经费项目(nyhyzx07-056)

摘　　要：To demonstrate the expression profiling of Xanthomonas oryzae pv.oryzae(Xoo) in vitro and in planta,DNA microarrays of 371 genes potentially associated with pathogenicity and virulence were used to compare the transcriptional level alteration of the wild-type strain PXO99A and gene deletion mutants ΔgacAxoo and ΔfleQxoo of Xoo grown in the rich medium NBY vs.hrp-inducing minimal medium XOM2 or leaf tissues of rice.Results indicated that 17 and 38 genes of PXO99A were differentially expressed in XOM2 and the leaf tissues of rice relative to NBY,respectively.Twenty-eight genes of ΔgacAxoo grown in XOM2 and 12 genes of ΔfleQxoo in NBY were differentially expressed relative to PXO99A.The identification of differentially-expressed genes,GacAxoo-and FleQxoo-regulons and novel candidate genes of Xoo strains would provide us the target genes for further functional analysis in pathogenesis of Xoo.To demonstrate the expression profiling of Xanthomonas oryzae pv.oryzae(Xoo) in vitro and in planta,DNA microarrays of 371 genes potentially associated with pathogenicity and virulence were used to compare the transcriptional level alteration of the wild-type strain PXO99A and gene deletion mutants ΔgacAxoo and ΔfleQxoo of Xoo grown in the rich medium NBY vs.hrp-inducing minimal medium XOM2 or leaf tissues of rice.Results indicated that 17 and 38 genes of PXO99A were differentially expressed in XOM2 and the...

关 键 词：Xanthomonas oryzae pv.oryzae growth in vitro and in planta DNA microarrays pathogenicity gene differentially-expressed genes 

分 类 号：S435.111.31[农业科学—农业昆虫与害虫防治]