河南省小麦黑胚病优势病原菌链格孢菌(Alternaria spp.)遗传多样性分析  

Analysis on genetic diversity of the dominant pathogens of wheat black point Alternaria spp. in Henan

作　　者：郜瑞敏[1] 袁虹霞[1] 邢小萍[1] 代君丽[1] 于巧丽[1] 李洪连[1] 

机构地区：[1]河南农业大学植物保护学院,郑州450002

出　　处：《植物病理学报》2009年第6期569-577,共9页Acta Phytopathologica Sinica

基　　金：国家粮食丰产科技工程河南课题(2006BAD02A07-1);中国科学院知识创新工程重大项目(KSCX1-YW-09);河南省科技攻关重点项目(0423011400)

摘　　要：采用RAPD技术对分离自河南省各地的43株小麦黑胚病优势病原菌(Alternariaspp.)菌株进行了遗传多样性分析。17个随机引物共扩增出151条清晰的DNA条带,所扩增出的DNA条带均为多态带,说明河南省小麦黑胚病菌存在着丰富的遗传多样性。利用NTSYS软件进行了病原菌的聚类分析,结果表明,河南省小麦黑胚病菌主要有2个种,即Alter-naria alternata和A.tenuissima,种间的遗传相似系数的变化幅度为0.62～0.92。来自同一个地区的小麦黑胚病菌菌株基本上聚在了一起,表现出很近的亲缘关系,来自不同地区之间的菌株也可以交叉聚类。病原菌遗传多样性分析进一步验证了形态学的鉴定结果。The genetic diversity of 43 Alternaria spp.isolates from the wheat black point of six representative areas in Henan Province was analyzed by RAPD.151 RAPD markers were obtained,which were amplified by 17 random primers,with 100% polymorphisms.It showed that rich genetic diversity of the pathogens of wheat black point was existed in Henan Province.The results of NTSYS cluster analysis showed that Alternaria alternata and A.tenuissima were the main pathogens of wheat black point,and the similarity coefficient...

关 键 词：小麦黑胚病 遗传多样性 链格孢 RAPD 

分 类 号：S435.121[农业科学—农业昆虫与害虫防治]