葡萄糖浓度波动对单核细胞表达白介素6的影响  

Effect of glucose fluctuation on IL-6 produced by Monocytes

作　　者：宋华静[1] 杨利波[2] 亓文波[2] 

机构地区：[1]泰山疗养院内科,山东泰安271000 [2]泰山医学院附属泰安医院,山东泰安271000

出　　处：《山东大学学报（医学版）》2009年第8期18-20,共3页Journal of Shandong University:Health Sciences

摘　　要：目的通过检测不同浓度葡萄糖及葡萄糖浓度波动对单核细胞产生白介素6(IL-6)的影响,探讨血糖波动在糖尿病大血管病变发生机制中的作用。方法实验所采用的细胞为THP-1细胞株,根据葡萄糖浓度及其变化分为对照组、高葡萄糖组、葡萄糖浓度波动组和渗透压控制组。将细胞接种于六孔培养板,每12 h换液1次,留取上清液。72 h后将每次留取的上清液等量混匀后采用酶联免疫法测定IL-6浓度,比较各组差异有无统计学意义。结果高葡萄糖组IL-6浓度明显高于对照组(P=0.000),葡萄糖浓度波动组高于高葡萄糖组(P=0.000),渗透压控制组介于高葡萄糖组和葡萄糖浓度波动组之间。结论葡萄糖浓度波动较单纯高糖更能促进单核细胞分泌IL-6,提示葡萄糖浓度波动有更强的细胞毒性。Objective To investigate the effect of fluctuation of glucose in diabetic macroangiopathy.Methods The THP-1cell strain,was randomly divided into the control group,the high glucose group,the fluctuating glucose concentration group and the osmotic pressure control group.Cells were incubated in a six-bore board,the culture fluid was changed every 12 h and the clean supernatant liquid was collected.The levels of IL-6 were detected by euzymelinked immunosorbent assay after 72 h.Result The level of IL-6 in the hi...

关 键 词：单核细胞 葡萄糖 浓度波动 白介素6 

分 类 号：R541.4[医药卫生—心血管疾病]