碘缺乏和甲状腺功能减退对大鼠仔鼠内嗅皮质钙调蛋白表达的影响  

Effect of Iodine Deficiency and Hypothyroidism on Calmodulin Expression in Entorhinal Cortex of Rat Pups

作　　者：侯怡[1] 魏薇[1] 董静[1] 王毅[1] 陈杰[1] 

机构地区：[1]中国医科大学公共卫生学院劳动卫生与环境卫生学教研室,沈阳110001

出　　处：《中国医科大学学报》2009年第11期804-806,共3页Journal of China Medical University

基　　金：国家自然科学基金资助项目(30500400)

摘　　要：目的观察碘缺乏和甲状腺功能减退损伤内嗅皮质对钙调蛋白(CaM)表达的影响。方法健康2月龄雌性Wistar大鼠,交配妊娠后,取孕鼠28只,按体质量随机分成对照组、甲状腺功能减退组和碘缺乏组,甲状腺功能减退组根据饮水中含丙基硫尿嘧啶(PTU)剂量分为5ppm组和15ppm组,每组7只孕鼠。分别于仔鼠出生后7,14,21,28,42d(PN7、PN14、PN21、PN28和PN42),每组随机取5只仔鼠,灌流固定大脑,进行组织病理切片和免疫组化染色,观察分析内嗅皮质钙调蛋白表达。结果PN14、PN21、PN28和PN42时,15ppm组和碘缺乏组仔鼠内嗅皮质钙调蛋白表达显著低于对照组(P<0.05)。PN7时,各组间钙调蛋白表达无显著差异。结论碘缺乏和甲状腺功能减退可下调大鼠仔鼠内嗅皮质钙调蛋白表达。Objective To observe the effect of iodine deficiency and hypothyroidism on calmodulin(CaM)expression in entorhinal cortex of rat pups.Methods A total of 28 pregnant Wistar rats were randomly divided into control group(n=7),hypothyroidism group(n=14),and iodine deficiency group(n=7).The rats of hypothyroidism group were subdivided into 5 ppm propylthiouracil(PTU)group(receiving 5 ppm of PTU,n=7)and 15 ppm PTU group(receiving 15 ppm of PTU,n=7),respectively.Five rat pups from each group were killed on postnat...

关 键 词：碘缺乏 甲状腺功能减退 内嗅皮质 钙调蛋白 

分 类 号：R581.2[医药卫生—内分泌]