p16^(INK4a)基因在CLD新生鼠肺成纤维细胞中的动态表达  

Dynamic Expression of p16^(INK4a) Gene in Lung Fibroblasts of Neonatal Rats with Chronic Lung Disease

作　　者：赵诗萌[1] 胡瑜[2] 薛辛东[2] 胡畔[3] 

机构地区：[1]中国医科大学附属第一医院新生儿科,沈阳110001 [2]中国医科大学附属盛京医院儿科,沈阳110004 [3]中国医科大学第91期临床医学系,沈阳110001

出　　处：《中国医科大学学报》2009年第11期810-813,共4页Journal of China Medical University

基　　金：国家自然科学基金资助项目(30672253)

摘　　要：目的探讨p16INK4a基因在高氧致慢性肺疾病(CLD)新生鼠肺成纤维细胞中的动态表达。方法足月新生SD大鼠于生后12h内分别持续吸入0.85高氧和空气,于3,7,14,21d随机处死动物后,进行肺成纤维细胞的原代培养,应用流式细胞仪测细胞周期;免疫组化、Western blot法检测p16INK4a蛋白表达;real-time PCR法检测p16INK4a mRNA含量变化。结果生后3d时2组p16INK4a蛋白及mRNA的表达无明显差异(P>0.05),生后7d时高氧组表达开始减少(P<0.05),14d、21d明显减少(P<0.01)。结论高氧可下调肺成纤维细胞p16INK4a基因的表达,使得其抑制细胞增殖的作用减弱,肺成纤维细胞过度增殖,最终导致肺纤维化的发生。Objective To explore the dynamic expression of p16INK4a gene in lung fibroblasts of neonatal rats with chronic lung disease(CLD).Methods Full-term newborn SD rats were continuously exposed to oxygen(0.85)or room air within 12 hours after birth.The rat pups were killed on postnatal days 3,7,14,and 21.The primary culture of lung fibroblasts were performed,and the cell cycle was detected by flow cytometry.The expressions of p16INK4a protein and mRNA in lung fibroblasts were determined by immunohistochemistry a...

关 键 词：高氧 早产儿慢性肺疾病 p16INK4a基因 肺成纤维细胞 

分 类 号：R722.1[医药卫生—儿科]